Observation on Correlation between Insulin Resistance Index and Blood Lipid in Obese Rats

doi:10.3969/j.issn.1674-5817.2018.06.003

Abstract

Abstract: Objective To obesrve the correlation between blood lipids and insulin resistance in obese rats induced by high-fat diet. Methods Male Sprague-Dawley rats were continuously fed with high fat diet supplemented with 36% fat energy for 12 weeks. The food intake, body weight were detected, Lee's index was calculated, serum which was collected by multiple time periods and the degrees of serum insulin, fasting blood glucose, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured, and insulin resistance index was calculated. Cluster analysis was used to screen successful insulin resistance rats and their differences in body weight, Lee's index, biochemical indicators compared to normal groups were analysed. Result Rats induced by high-fat diet developed obesity after 4 weeks with higher levels of body weight and Lee's index (P<0.01, P<0.05). Sixteen rats producing insulin resistance were selected by cluster analysis based on the 12-week insulin resistance index of obese rats. After statistical analysis, 16 rats developed insulin resistance after 8 weeks, with lower levels of TC and HDL-C as compared with the normal group (P<0.05). But there was no significant change in FBG, TG and LDL-C contents. Conclusion Obese rats induced by 36% fat-energy high-fat diet began to produce insulin resistance with about 50% of the obese rats at the end of the 8th week and the lipid metabolism were significantly disordered in insulin resistance rat.

Key words: High-fat diet, Obesity, Insulin resistance

CLC Number:

Q95-33

SHENG Yi-xuan, ZENG Guo-wei, YAO Liang-liang, LI Bing-tao, JIANG Li, ZHANG Qi-yun, XU Guo-liang. Observation on Correlation between Insulin Resistance Index and Blood Lipid in Obese Rats[J]. Laboratory Animal and Comparative Medicine, 2018, 38(6): 417-421.

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