Abstract: Objective To screen the high-glucose-fat diet for inducing obesity in rats and mice. Methods The rats were fed with five kinds of modified high fat diet for eight weeks, the body weight、Lee's index、organ coefficient、abdominal fat index、blood lipid and blood glucose of the rats and mice were determined. The mice were fed with high fat diet C for 8 weeks ,and the corresponding data were determined also. Results Compared with the control group, group C rats fed with high fat diet increase significantly in body weight, Lee's index, organ coefficient of the liver, kidney, spleen and testicular, abdominal fat index, triglyceride, cholesterol, blood sugar (P<0.05). Compared with the control group, group C mice fed with high fat diet increased significantly in body weight, organ coefficient of heart, liver, spleen, and kidney, abdominal fat index, triglyceride and blood sugar (P<0.05), but no significant change (P>0.05) in Lee's index, cholesterol and testicular organ coefficient. Conclusion High-fat diet can cause mice and rats nutritional obesity, and blood sugar was significantly increased. High fat diet formula composed of 15% total fat and 24% soybean meal is more reasonable, and the effects on obesity in rat was more pronounced than in mice.

Key words: Nutritional obesity, High fat diet, Model, Comparison