Generation and Phenotyping Analysis of Lep Gene Knockout Mice

doi:10.3969/j.issn.1674-5817.2017.05.002

Abstract

Abstract: Objective To provide mouse models for obesity and diabetes research and drug development, the Leptin (Lep) gene knockout mouse model was established and analyzed. Methods The Lep gene knockout mouse model was established by using embryonic stem cell and homologus recombination technology. Body weight and plasma glucose were measured, and the three genotypes of Lep gene knockout mice were sacrificed for detection of the serum biochemistry parameters. All the data generated from Lep gene knockout mice were compared with age-matched heterozygote(He) and wild type (WT) mice in statistics. Pathological changes of hepatic and kidney tissues between Lep gene knockout and WT mice were observed under microscope. Results The Lep gene knockout mouse model was established. Preliminarily phenotypic analysis show that Lep gene knockout mice develop obesity and hyperinsulinemia, hyperglycemia, lipid metabolism disorder, liver dysfunction .From the pathological analysis, the hepatocyte swelling and macro vacuolar steatosis were observed. Conclusion The Lep gene knockout mouse model was established and it could be used for obesity and diabetes research and drug development.

Key words: Leptin (Lep) gene, Gene knockout mice, Obesity, Type 2 Diabetes

CLC Number:

Q95-33

CHI Jun, GONG Hui, HE Yue-wei, WAN Ying-han, FEI Jian, KUANG Ying. Generation and Phenotyping Analysis of Lep Gene Knockout Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(5): 344-351.

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