Abstract: Objective To study the effect of childhood obesity induced by high fat diet on the metabolism of glucose and lipid. Methods Thirty male SD rats (21-day-old) were randomly divided into 2 groups, and were given basic diet or high fat diet, respectively. At the 60 days old, the insulin tolerance test of rats of rats was proceeded, the body length and weight the rats were measured accurately, and the blood was collected for the measurement of biochemical markers. After the rats were sacrificed, the livers of rats were collected, sliced and stained for pathological observation. Result The obesity rat model induced by high fat diet was established successfully. The serum level of triglyceride (TG) and and total cholesterol (TC) in obese rats were higher than those of non-obese rats (P<0.05), but there were no differences in alanine aminotransferase (ALT), fasting blood glucose (FBG), high-density lipoprotein cholesterol (HDL-C), and insulin (Ins) between groups (P>0.05). At each time point, there was no difference on the insulin tolerance test. Under the microscope, there were numbers of fat bubbles in liver cells of young obese rats. Conclusion Compared with the non-obese young rats, the metabolism of lipids (TG, TC) of the obese young rats was disordered, the level of FBG and ALT showed an increasing trend, the level of HDL-C and Ins showed a decreasing trend. Obesity induced by high fat diet can lead to fatty liver of young rats.

Key words: High fat diet, Obesity young rats, Lipid metabolism disorders