Abstract: Objective To investigate the effect of high fat diet on Type 2 dialbetes mellitus (T2DM) and obesity, the model of T2DM was established by using prolonged high-fat diet (HFD) feeding on C57BL/6 mice. Methods C57BL/6 mice were fed with 60F10S diet for 12 weeks, the fasting blood-glucose (FBG) and animal body weight were monitored at six different time points. Moreover, oral glucose tolerance test (OGTT) was carried out to detect the blood glucose regulation ability and reflect the β cells dysfunction. Results Compare to the control group, the FBG had significantly increased from fourth week (P<0.001), while body weight grew steadily until the 12th week to formation of obesity (P<0.001). Correlation analysis reveals that the changes of FBG and body weight were correlated (P<0.001). After twelve weeks HFD feeding, the area under the curve of the OGTT (in mmol·h^-1·L^-1) was extraordinarily enlarged which means that the mice had poorer ability of blood glucose regulation (P<0.001). Conclusion Obese models of T2DM can be successfully induced by 12 weeks’ HFD feeding in C57BL/6 mice. As the simulation of high-fat diet in modern life, this model can reproduces the T2DM caused by diet disorders, and provides a powerful tool for the study of diabetes.

Key words: C57BL/6 mice, High-fat diet (HFD), Type 2 diabetes mellitus (T2DM)