Helicobacter hepaticus Infection Promotes High Fat Diet-induced Non-alcoholic Fatty Liver Disease in Mice

doi:10.12300/j.issn.1674-5817.2020.181

Abstract

Abstract: Objective To investigate the effect of Helicobacter hepaticus (H.h) on non-alcoholic fatty liver disease induced by a high fat diet (HFD). Methods Twenty 6-week-old male BALB/c mice were randomly divided into four groups: control group, H.h group, HFD group and H.h+HFD group. The H.h+HFD group was fed a HFD after H.h infection. After 12 weeks of feeding, the levels of triglyceride (TG), alanine transaminase (ALT) and aspartate aminotransferase (AST) were measured. Besides, the liver index was calculated, and oil red O staining, Sirius red staining, hematoxylin-eosin staining, real-time fluorescence quantitative PCR (RT-PCR) and immunohistochemistry were conducted to evaluate liver pathological changes in the liver samples. Results The serum levels of TG, ALT and AST in the H.h+HFD group were significantly higher than those in the control group, H.h group and HFD group (all P<0.05). The livers of mice in the H.h+HFD group showed multiple lesions, fat accumulation, ballooning degeneration and necrosis, collagen fiber deposition. The livers of mice in the H.h group exhibited inflammatory cell aggregation and a small amount of collagen fiber deposition, whereas the livers of mice in the HFD group showed lipid droplets accumulation, steatosis and a small amount of collagen fiber deposition. Moreover, the mRNA levels of interleukin (IL)-6, IL-1?, transforming growth factor-? (TGF-?) and tumor necrosis factor-〈 (TNF-〈) were significantly elevated in the livers of mice in the H.h+HFD group compared to the other three groups (all P<0.05). The expressions of alpha-smooth muscle actin (〈-SMA) and collagen typeⅠ(CollagenⅠ) were significantly increased in the H.h+HFD group. Conclusion H.h infection can promote non-alcoholic fatty liver disease induced by feeding HFD to mice.

Key words: Helicobacter hepaticus, Non-alcoholic fatty liver disease, High-fat diet, Liver fibrosis, Mice

CLC Number:

R-332
Q95-33

SHEN Chen, WU Zhihao, YIN Jun, ZHU Liqi, ZHANG Quan. Helicobacter hepaticus Infection Promotes High Fat Diet-induced Non-alcoholic Fatty Liver Disease in Mice[J]. Laboratory Animal and Comparative Medicine, 2021, 41(2): 122-130.

Figures/Tables 5

References

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