Comparison on Two Models of Epiphyseal Plate Injury in Rat

doi:10.3969/j.issn.1674-5817.2015.05.002

Abstract

Abstract: Objective To compare two methods for constructing animal models of epiphyseal plate injury, and therefore to learn more about the disease of epiphyseal plate injury. Methods Fourty-five Wistar rats aged between 4-5 weeks were divided into three groups. Group A was control group, without operation; Group B was direct-injury group, in which the upper part of right tibial epihpyseal plate were cut; Group C was manual-rotation group, in which the knees of rats were grabbed valgusly. The rats were sacrificed after 4, 10 and 21days of operation. Micro-CT scanning and histological assessments were done. The differences between the length of left and right tibia, the width of tuberositas of left and right tibiae, and the minimum distance between tibia and fibula were examined by CTAn(Comprehensive TeX Archive Network) software. Results Micro-CT scanning and histological assessments showed that, after 4 days of operation, the width of epiphyseal plate were larger in Group B and Group C, immature chondrocytes were found in Group B, and the bone mineral density around operation site were lower than control in Group B. After 10 days of operation, the epiphyseal plate became narrower in Group B, and the bone mineral density around operation site appeared to be lower than normal in Group C. After 21 days of operation, the bone bridge was found in Group B, and only small new-born sclerotin appeared in Group C. According to the length of tibia, the width of tibial tuberosity, and the maximal interval between tibia and fibula, all of these values in Group B and Group C were significantly higher than Group A (control group) (P<0.05); but the values after operation of 21 days of Group B were significantly higher than those of Group B (P<0.05), other values were not significantly different between the two groups (P>0.05). Conclusion It is proved that the method of direct-injury is a much better one for constructing rat models of epiphyseal plate injury. This method has good stability and variation which more proper for studying disease of epiphyseal plate injury.

Key words: Animal model, Micro-CT, Epiphyseal plate injury

CLC Number:

Q95-33

JING Jin-zhu, LI Qing-hai, LV Xue-min, LIANG Zhen, WANG Meng. Comparison on Two Models of Epiphyseal Plate Injury in Rat[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 351-355.

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