Abstract: Objective To optimize the method for establishing an animal model of atrial fibrillation(AF) based on self-designed implantable telemetry and stimulator. Method First, the anesthetic protocols were refined. Thirty New Zealand rabbits, half males and half females, were divided into three groups. Group A, pentobarbital sodium only, Group B, xylazine with diazepam and lidocaine, and Group C, xylaxine with midazolam and lidocaine. Second, the location of recording electrodes were optimized by using a needle to connect to ECG collect electrode and the needle was punctured to the different location of the rabbit chest subcutaneous to analyze the electrocardiogram before choose the best location. Third, the stimulating electrode was refined and the method of operation was improved. Results At the anesthesia, the heart rate and breath rate of Group A were depressed more than Group B and Group C, first anesthesia time of Group B was longest, complemental anesthesia times of Group C was more than Group A and Group B. The best electrode location could prove good ECG record . Refined stimulating electrode could improve the effect of stimulation. Reformed operation method could simplify the operation procedure. Conclusion The refined method for establishing an animal model of atrial fibrillation used by lmplantable telemetry and stimulator was feasible, and more conformance to animal welfare.

Key words: Atrial fibrillation(AF), Animal model, Rabbit, Refinement