Influence of Corneal Staining in Rabbits on the Evaluation of Eye Irritation Test Results

doi:10.12300/j.issn.1674-5817.2022.169

Abstract

Abstract:

Objective To observe the influence of the staining phenomenon after fluorescein sodium staining on eye irritation in normal rabbits. Methods In the experimental rabbit eye irritation test conducted with sodium chloride eye drops, Siwei Zhenceng Bingpeng eye drops, sodium hyaluronate eye drops, sodium cromoglycate eye drops, and compound aspartate eye drops (4 in each group, half male and half female), the left eyes of rabbits were administered normal saline (self-negative control) and the right eyes were administered the experimental medicine; the eyes were stained with 1% sodium fluorescein, and eye irritation was observed and scored using slit lamp microscope for 31 days. Morphological changes of corneal epithelial staining were recorded and the incidence of staining was calculated. After the observation, the eyeballs and Hasselblad glands were examined histopathologically, and the staining rate of the left eye was compared with that of the right eye which was administered the corresponding medicine. Results Neither eye had any irritation symptoms; the scores were 0, and the total incidences of corneal staining were 3% (left) and 1% (right), respectively. There was no significant difference between the two groups (P > 0.05). Corneal epithelial staining showed single-spot staining, scattered dot, localized, or large areas of fusion staining. No histopathological changes were found in the eyeballs or Hasselblad glands, and the results were evaluated as non-irritative. Conclusion The irregularity of corneal epithelial staining in rabbits did not influence the results of the ocular irritation test.

Key words: Rabbits, Cornea, Staining, Eye irritation test, Result evaluation

CLC Number:

R-332

Honghua XU, Tian JIN, Hai WANG, Mengying SHEN, Rui WANG, Yijia ZHOU, Ying TAN. Influence of Corneal Staining in Rabbits on the Evaluation of Eye Irritation Test Results[J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 307-313.

Figures/Tables 4

References 16

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刺激部位 Site of irritation	眼刺激反应 Reaction to eye irritation	分值 Score
角膜Cornea	无混浊	0
	散在或弥漫性混浊，虹膜清晰可见	1
	半透明区易分辨，虹膜模糊不清	2
	出现灰白色半透明区，虹膜细节不清，瞳孔大小勉强可见	3
	角膜不透明，虹膜无法辨认	4
虹膜Iris	正常	0
	皱褶明显加深、充血、肿胀，角膜周围轻度充血，瞳孔对光仍有反应	1
	出血，肉眼可见坏死，对光无反应（或其中一种）	2
结膜Conjunctiva	血管正常	0
	血管充血呈鲜红色	1
	血管充血呈深红色，血管不易分辨	2
	弥漫性充血呈紫红色	3
	无水肿	0
	轻微水肿（含眼睑）	1
	明显水肿伴部分眼睑外翻	2
	水肿至眼睑近半闭合	3
	水肿至眼睑超过半闭合	4
	无分泌物	0
	少量分泌物	1
	分泌物使眼睑和睫毛潮湿或黏着	2
	分泌物使整个眼区潮湿或黏着	3
最大总积分Maximum total integral value		16

刺激部位 Site of irritation	眼刺激反应 Reaction to eye irritation	分值 Score
角膜Cornea	无混浊	0
	散在或弥漫性混浊，虹膜清晰可见	1
	半透明区易分辨，虹膜模糊不清	2
	出现灰白色半透明区，虹膜细节不清，瞳孔大小勉强可见	3
	角膜不透明，虹膜无法辨认	4
虹膜Iris	正常	0
	皱褶明显加深、充血、肿胀，角膜周围轻度充血，瞳孔对光仍有反应	1
	出血，肉眼可见坏死，对光无反应（或其中一种）	2
结膜Conjunctiva	血管正常	0
	血管充血呈鲜红色	1
	血管充血呈深红色，血管不易分辨	2
	弥漫性充血呈紫红色	3
	无水肿	0
	轻微水肿（含眼睑）	1
	明显水肿伴部分眼睑外翻	2
	水肿至眼睑近半闭合	3
	水肿至眼睑超过半闭合	4
	无分泌物	0
	少量分泌物	1
	分泌物使眼睑和睫毛潮湿或黏着	2
	分泌物使整个眼区潮湿或黏着	3
最大总积分Maximum total integral value		16

组别 Test group	观察部位 Observed position	每天发生率/% Daily incidence/%											总发生率/% Total incidence/%
组别 Test group	观察部位 Observed position	D1	D3	D9	D10	D12	D17	D18	D20	D24	D28	D31	总发生率/% Total incidence/%
氯化钠滴眼液组（n=4） Sodium chloride eye drops	左眼	0	0	25	0	25	0	0	25	25	0	0	3.2
氯化钠滴眼液组（n=4） Sodium chloride eye drops	右眼	0	25	0	0	0	25	0	0	0	25	0	2.4
四味珍层冰硼滴眼液组（n=4） Siwei Zhenceng Bingpeng eye drops	左眼	0	0	0	25	25	0	0	25	0	0	0	2.4
四味珍层冰硼滴眼液组（n=4） Siwei Zhenceng Bingpeng eye drops	右眼	0	25	0	0	0	0	25	0	0	0	0	1.6
玻璃酸钠滴眼液组（n=4） Sodium hyaluronate eye drops	左眼	0	0	25	0	25	0	0	25	0	0	0	2.4
玻璃酸钠滴眼液组（n=4） Sodium hyaluronate eye drops	右眼	0	0	0	0	0	25	0	0	0	0	0	0.8
色甘酸钠滴眼液组（n=4） Sodium cromoglycate eye drops	左眼	0	0	0	0	25	0	0	0	25	0	25	2.4
色甘酸钠滴眼液组（n=4） Sodium cromoglycate eye drops	右眼	0	0	0	0	0	0	25	0	0	0	0	0.8
复方门冬维甘滴眼液组（n=4） Compound aspartate eye drops	左眼	0	0	0	25	0	0	0	25	0	0	25	2.4
复方门冬维甘滴眼液组（n=4） Compound aspartate eye drops	右眼	0	0	0	0	0	0	0	0	0	25	0	0.8
全部实验兔（n=20） All experimental rabbits	左眼	0	0	10	10	20	0	0	20	10	0	10	3.0
全部实验兔（n=20） All experimental rabbits	右眼	0	10	0	0	0	10	10	0	0	10	0	1.0

组别 Test group	观察部位 Observed position	每天发生率/% Daily incidence/%											总发生率/% Total incidence/%
组别 Test group	观察部位 Observed position	D1	D3	D9	D10	D12	D17	D18	D20	D24	D28	D31	总发生率/% Total incidence/%
氯化钠滴眼液组（n=4） Sodium chloride eye drops	左眼	0	0	25	0	25	0	0	25	25	0	0	3.2
氯化钠滴眼液组（n=4） Sodium chloride eye drops	右眼	0	25	0	0	0	25	0	0	0	25	0	2.4
四味珍层冰硼滴眼液组（n=4） Siwei Zhenceng Bingpeng eye drops	左眼	0	0	0	25	25	0	0	25	0	0	0	2.4
四味珍层冰硼滴眼液组（n=4） Siwei Zhenceng Bingpeng eye drops	右眼	0	25	0	0	0	0	25	0	0	0	0	1.6
玻璃酸钠滴眼液组（n=4） Sodium hyaluronate eye drops	左眼	0	0	25	0	25	0	0	25	0	0	0	2.4
玻璃酸钠滴眼液组（n=4） Sodium hyaluronate eye drops	右眼	0	0	0	0	0	25	0	0	0	0	0	0.8
色甘酸钠滴眼液组（n=4） Sodium cromoglycate eye drops	左眼	0	0	0	0	25	0	0	0	25	0	25	2.4
色甘酸钠滴眼液组（n=4） Sodium cromoglycate eye drops	右眼	0	0	0	0	0	0	25	0	0	0	0	0.8
复方门冬维甘滴眼液组（n=4） Compound aspartate eye drops	左眼	0	0	0	25	0	0	0	25	0	0	25	2.4
复方门冬维甘滴眼液组（n=4） Compound aspartate eye drops	右眼	0	0	0	0	0	0	0	0	0	25	0	0.8
全部实验兔（n=20） All experimental rabbits	左眼	0	0	10	10	20	0	0	20	10	0	10	3.0
全部实验兔（n=20） All experimental rabbits	右眼	0	10	0	0	0	10	10	0	0	10	0	1.0

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