Evaluation of Sp3111 RNAi Transgenic Mice Model and Outcome of in vitro Fertilization with Spermatozoa from Sp3111 RNAi Transgenic Mice

doi:10.3969/j.issn.1674-5817.2011.02.002

Abstract

Abstract: Objective To evaluate application value of Sp3111 RNAi transgenic mice model and to study the function of the sp3111 protein in fertilization and early embryo development. Methods ①real-time quantitative PCR was used to analysis the expression of sp3111 mRNA both in F1 and F4 generation, and comparing the suppression efficiency of RNAi between the two generations. ②Sperm samples collected from male Sp3111 RNAi transgenic mice(F4 generation) and from wild type mice as control group followed by IVF. The two groups were observed for the rates of fertilization and embryo fragmentation. Results ①No significant difference was found between F1 and F4 generation in suppression efficiency of RNAi PŁľ0.05. ②The rates of normal and fragmented embryos of the experimental group were 34.48% and 46.21%, respectively,significantly different from those of the control group (P<0.05). Conclusion ①the suppression efficiency of RNAi in Sp3111 RNAi transgenic mice could be transmitted stably to F4 generation. ②The low expression of sp3111 gene remarkably affected fertilization and early embryo development in mice.

Key words: Transgenic RNAi mice, Genetic stability, In vitro fertilization, Real-Time PCR

CLC Number:

Q95-33

HUA Ye, JIA Xiao-feng, HUA Min-min, YUAN Yao, FAN Li-qiang, SHI Hui-juan. Evaluation of Sp3111 RNAi Transgenic Mice Model and Outcome of in vitro Fertilization with Spermatozoa from Sp3111 RNAi Transgenic Mice[J]. Laboratory Animal and Comparative Medicine, 2011, 31(2): 83-86.

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