Detection of Eimeria Stiedai by Real-Time PCR

doi:10.3969/j.issn.1674-5817.2017.06.005

Abstract

Abstract: Objective To develop the method for quantitatively detecting Eimeria stiedai (E. stiedai) by Real-Time PCR (RT-PCR). Methods Specific primers were designed and synthesized according to a part of the sequence of ITS1 region of E. stiedai. The recombinant plasmid was constructed to established the standard curve of RT-PCR. The specificity, sensitivity and reproducibility of the RT-PCR method were performed. Result The method was specific and sensitive for detection of E. stiedai. Meanwhile, the sensitivity of the present method could detect the DNA of one E. stiedai and the variation coefficients of intra-assay and inter-assay were less than 2.0% respectively. Conclusion The established RT-PCR is a specific, sensitive and reliable method for the quantitative detection of E. stiedai.

Key words: E. stiedai, Real-Time PCR(RT-PCR), Detection

CLC Number:

S858.28Q95-33

WEN Fu-li, ZHENG He-ping, DANG Yuan, XUE Lai-en, ZHANG Shi-lan. Detection of Eimeria Stiedai by Real-Time PCR[J]. Laboratory Animal and Comparative Medicine, 2017, 37(6): 448-454.

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