A Comprehensive Method of Strain Conservation Mediated by Spermatozoa in Mice

doi:10.3969/j.issn.1674-5817.2012.02.013

Abstract

Abstract: Objective To establish a comprehensive system for mouse strain conservation through natural mating, sperm cryopreservation and assisted reproductive technology. Methods Mouse fresh epididymal sperm were collected by vasectomy of unilateral testis and the vasectomized mice were naturally mated for breeding. If no satisfactory results obtained from natural breeding, then the cryopreserved sperm were thawed for In Vitro Fertilization (IVF). If no results obtained, Intracytoplasmic Sperm Injection (ICSI) was used to reconstructed embryos and which were finally transferred into pseudopregnant recipient female mouse. Results The results show that parts of mice vasectomized unilateral testis such as BDF1 and C57BL/6J mice could be used for natural breeding. Among the remaining sterile males, only ICR mice could be preserved through IVF while ICSI technology was the last approach to save GFP mice with C57BL/6J background. Conclusion The genetic background of mouse sperm cryopreservation was a key factor in IVF and setup a comprehensive system, which could achieve the purpose of strain conservation while mouse strains could be utilized at maximum extent.

Key words: Mouse, Vasectomy of Unilateral Testis, In Vitro Fertilization, Intracytoplasmic Sperm Injection, Strain conservation

CLC Number:

Q95-33

LI Dun-Gao, JIANG Man-Xi, CHEN Xue-Jin, LI He-Ping. A Comprehensive Method of Strain Conservation Mediated by Spermatozoa in Mice[J]. Laboratory Animal and Comparative Medicine, 2012, 32(2): 142-145.

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