Abstract: Objective To establish a rapid detection method for helicobacter rodentium.Method A pair of specific primers was designed according to the gene sequence of Helicobacter rodentium 16S rRNA.The method of SYBR Green Ⅱ real-time PCR was established.Results The standard curve cycle threshold had a good linear relationship with the template concentration in the range of 10² copies /µL to 10⁷ copies /µL concentration.This method has good sensitivity,only specific amplification of Helicobacter rodentium,could detect 30 copies /µL,the coefficient of variation (CV) was less than 2% within and between groups with good reproducibility.Conclusion The method of SYBR Green Ⅱ real-time PCR was established for the identification of Helicobacter rodentium.

Key words: Helicobacter rodentium, SYBR Green Ⅱ, Real-time PCR