Application of In Vitro Fertilization in Rat Embryos Cryopreservation

doi:10.3969/j.issn.1674-5817.2015.04.007

Abstract

Abstract: Objective To investigate the feasibility of in vitro fertilization (IVF) technique in rat embryos cryopreservation. Methods IVF-20 was used as rat sperm capacitation and fertilization medium, mR1ECM as a culture solution for IVF fertilized egg of SD rats; 2-cell embryo were obtained by flushing as a control, and well-developed 2- embryos were cryopreserved, after a week the thawed embryos were transplanted to recipients of same strain rats. Results A total of 463 and 588 embryos were separately collected from IVF group and control group, the development rate of normal 2-cell embryos was 65.01% (301/463) and 63.94% (376/588) respectively, which showed no significant difference in the fertilization rate (P>0.05). In the 150 embryos in each group, morphologically normal embryos were 132 (88.00%) and 130 (86.66%) after the recovery, there was no significant difference (P>0.05); Surviving embryos were transplanted into five recipients of same strain rat, and newborn were 45 and 22. The litter rate (2.14% vs16.92%) showeda significant difference between the two groups (P<0.05). Conclusion IVF technology of rat is feasible in the embryo cryopreservation and breed preservation. It provides technical supports for cryopreservation of genetic manipulated rats in future.

Key words: Rat, Superovulation, In vitro fertilization(IVF), Embryo cryopreservation

CLC Number:

Q95-33

ZHOU Sheng-lai, WANG Wei, YU Yang, YANG Wei, ZHANG Mei-ying, ZHENG Zhi-hong. Application of In Vitro Fertilization in Rat Embryos Cryopreservation[J]. Laboratory Animal and Comparative Medicine, 2015, 35(4): 298-301.

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