Influence of Reduced Glutathione Added Medium on in vitro Fertilization in C57BL/6J Mouse

doi:10.3969/j.issn.1674-5817.2016.03.010

Abstract

Abstract: Objective To study the influence of reduced glutathione (GSH) in fertilization medium on the in vitro fertilization (IVF) in C57BL/6J mouse. Methods The fertilization medium without reduced glutathione was prepared as control group, and the different concentrations of GSH (0.25 mmol/L, 0.50 mmol/L, 0.75mmol/L, 1.00mmol/L, 1.25mmol/L and 1.50mmol/L) were added to the fertilization medium during IVF, which were divided into 6 experimental groups, to investigate its effect on the fertilization and early embryo development of C57BL/6J inbred mice. In addition, 5 strains of genetically engineered mice having a genetic background of C57BL/6J were chosen to verify the influence of GSH. Results Compared with the control, GSH increased the fertilization rate in a dose-dependent manner. Statistically significant increased fertilization was first observed at 0.5 mmol/L GSH (P<0.05), reaching maximum values at 1.25 mmol/L(P<0.01). The blastocyst developmental rates have no significant difference from that of the control group (P>0.05). Fertilization and development rates of the genetically engineered mice were similar to those of C57BL/6 inbred mice. Conclusion In a certain range of concentration of GSH during the IVF procedure, it can enhance the fertility of C57BL/6J mouse and the genetically engineered mice having a genetic background of C57BL/6J.

Key words: Reduced glutathione(GSH), C57BL/6J mouse, In vitro fertilization (IVF)

CLC Number:

Q95-33

ZHAO Chen, ZHANG Shuang-qin, Wang Li-feng, LI Wei. Influence of Reduced Glutathione Added Medium on in vitro Fertilization in C57BL/6J Mouse[J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 208-211.

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