Analysis on Differential Genes Expression in Early Fetuses Derived from in vitro Fertilization Using Oocytes from Induced Diabetes Mouse

doi:10.3969/j.issn.1674-5817.2013.02.002

Abstract

Abstract: Objective To study the effect of maternal diabetes environment on early fetuses gene expression through in vitro Fertilization(IVF) technology. Methods Diabetes animal model was induced by a low dose of STZ in 6-8 weeks ICR female mice, and then the mice with hyperglycemia were superovulated. Two-cell embryos derived from in vitro fertilization were transferred into the oviduct of normal pseudopregnant ICR female mouse. Total RNA of 14 days’ fetuses were collected and detected by Agilent 2100 Bioanalyzer system. Through subsequent reversed transcription, label, hybridization, elution and microarray scann by Affymetrix Scanner 3000, finally raw data was read by Command Console Software 3.1. The experiment was repeated three times. Results 121 differentially expressed genes (P<0.05) were screened out. The expression level of 119 genes in the experimental group was less 0.5 times than that in the control group, and it shown more than 2-fold up regulation. Conclusion Maternal diabetes environment influenced the growth and development of the oocytes through up-regulating the metabolism-related genes and down-regulated development-relating genes and further influenced the growth and development of the early embryos.

Key words: Diabetes mouse, In vitro Fertilization(IVF), Microarray, Differential expression

CLC Number:

Q95-33

TAO Ling-yun, QIANG Su-jing, GAO Cheng, HU Jian-hua. Analysis on Differential Genes Expression in Early Fetuses Derived from in vitro Fertilization Using Oocytes from Induced Diabetes Mouse[J]. Laboratory Animal and Comparative Medicine, 2013, 33(2): 84-89.

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