Different Oocyte Collecting Time Impact on Quality of Oocyte,in Vivo Maturation Rate and in Vitro Fertilization Rate after Superovulation in KM Mice

doi:10.3969/j.issn.1674-5817.2017.05.012

Abstract

Abstract: Objective To improve the quality of oocyte, in vivo maturation rate and in vitro fertilization rate in KM mice. Methods One-hundred KM mice were super-ovulated, the changes of the oocyte nucleus and the first polar body position, the perivitelline space changes, oocyte morphology, in vivo maturation rates and in vitro fertilization rates were observed and calculated. Results The oocyte quality was best after 12 h、14 h of injection with human chorionic gonadotropins (hCG), and oocyte in vivo maturation rate and in vitro fertilization rate were the highest after 13 h. Conclusion Collection of oocyte after 13 h of superovulation was the best time for in vitro fertilization of KM mouse.

Key words: Mice, Superovulation, Collecting oocyte time, Oocyte quality, In vivo maturation rate, In vitro fertilization rate

CLC Number:

Q95-33

PENG Li-fan, ZHANG Xiao-jian, LIAO Mei-xu, LI Xiao-jie, YU Kun. Different Oocyte Collecting Time Impact on Quality of Oocyte,in Vivo Maturation Rate and in Vitro Fertilization Rate after Superovulation in KM Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(5): 405-409.

References

[1] Alexander M, Rudolf J.Mammalian nuclear transfer[J]. Dev Dyn, 2015, 8(235):2460-2469.
[2] Alcobia I, Dilao R, Parreira L.Spatial associations of centromeres in the nuclei of hematopoietic cells: evidence for cell-type-specific organizational patterns[J]. Blood, 2014, 21(95):1608-1615.
[3] Alcobia I, Quina AS, Neves H, et al.The spatial organization of centromeric heterochromatin during normal human lymphopoiesis: evidence for ontogenically determined spatial patterns[J]. Exp Cell Res, 2014, 24(290):358-369.
[4] Armstrong L, Lako M, Dean W, et al.Epigenetic modification is central to genome reprogramming in somatic cell nuclear transfer[J]. Stem Cells, 2015, 4(24):805-814.
[5] Cremer T, Cremer C.Chromosome territories, nuclear architecture and gene regulation in mammalian cells[J]. Nat Rev Genet,2014, 3(2):292-301.
[6] Cremer T, Cremer C.Rise, fall and resurrection of chromosome territories: a historical perspective of chromosome territories[J]. Eur Histochem, 2013, 5(50):161-176.
[7] Cremer T, Cremer C.Rise, fall and resurrection of chromosome territories: a historical perspective and resurrection of chromosome territories during the 1950s to 1980s. Part III. Chromosome territories and the functionalnuclear architecture: experiments and models from the 1990s to the present[J]. Eur Histochem, 2013, 3(50):223-272.
[8] Cremer M, Kupper K, Wagler B, et al.Inheritance of gene density-related higher order chromatin arrangements in normal and tumor cell nuclei[J]. Cell Biol, 2013, 5(162):809-820.
[9] Cremer T, Cremer M, Dietzel S.Chromosome territories functional nuclear landscape[J]. Curr Opin Cell Biol, 2016, 3(18):307-316.
[10] Grigoryev SA, Nikitina T, Pehrson JR, et al.Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence[J]. Cell Science, 2014, 5(117): 6153-6162.
[11] Yanagimachi R.Cloning: experience from the mouse and other animals[J]. Mol Cell Endocrin, 2014,7(187):241-248.
[12] Xie HL, Wang YB, Jiao GZ, et al.Effects of glucose metabolism during in vitro maturation on cytoplasmic maturation of mouse oocytes[J]. Sci Rep, 2016(6):20764.
[13] Taiyeb AM, Muhsen-Alanssari SA, Dees WL, et al.Improvement in in vitro fertilization outcome following in vivo synchronization of oocyte maturation in mice[J]. Exp Biol Med (Maywood), 2015, 240(4):519-526.
[14] Eppig JJ, O'Brien MJ, Wigglesworth K, et al. Effect of in vitro maturation of mouse oocytes on the health and lifespan of adult offspring[J]. Hum Reprod, 2009, 24(4):922-928.
[15] Zavareh S, Karimi I, Salehnia M, et al.Effect of in vitro maturation technique and alpha lipoic acid supplementation on oocyte maturation rate: focus on oxidative status of oocytes[J]. Int Fertil Steril, 2016, 9(4):442-451.
[16] 彭礼繁. 体细胞克隆小鼠胚胎的试验研究[D]. 沈阳: 沈阳农业大学, 2009.
[17] 刘海霞. 培养箱内的二甲苯, 甲醛对小鼠体外受精及胚胎发育的影响[D]. 唐山: 华北理工大学, 2016.
[18] 房丽娜. 培养箱中的乙醇和三氯甲烷浓度对小鼠体外受精及胚胎发育的影响[D]. 唐山: 华北理工大学, 2016.
[19] 宋姝君. 培养箱内的温度和PH值对小鼠体外受精及胚胎发育的影响[D]. 唐山: 华北理工大学, 2016.
[20] 左琴, 范涛, 张翠平, 等. 获能培养液等因素对遗传工程小鼠冻融精子体外受精率的影响[J]. 中国比较医学杂志, 2015, 7(5):31-35.