Analysis on Cyclooxygenase 2 Expression in Different Tissues from Spontaneous Diabetic Mongolian Gerbils

doi:10.3969/j.issn.1674-5817.2017.02.007

Abstract

Abstract: Objective To analyze Cyclooxygenase 2(COX-2) expression level in different tissues of diabetic gerbils and to explore the relationship of diabetes occurrence and development in Mongolian gerbils. Methods Different tissues including skeletal muscle, liver and kidney were collected from 6 diabetic gerbils and 6 control animals respectively. COX-2 mRNA and protein expression level in different tissues were separately detected by Real-time PCR and Western blotting. Results The Real-time PCR showed that compared with control animal, the mRNA expression of COX-2 in skeletal muscle had no obvious difference whereas it exhibited uptrend in liver and kidney. As the results of Western blotting, the difference of COX-2 in skeletal muscle was almost undetectable in diabetes group. The relative expression level in diabetic group exhibited uptrend compared with control in liver and kidney. And there was statistical significance in the kidney. Conclusions COX-2 was high expressed in liver and more remarkably in kidney of diabetic gerbils. It may illustrate that the effect of COX-2 mainly occurred in liver and kidney of diabetic gerbils.

Key words: Mongolian gerbils, Type 2 diabetes, Cyclooxygenase 2 (cox-2), Real-time PCR

CLC Number:

Q95-33

WANG Fei-fei, GONG Jing-jing, HUO Xue-yun, LU-jing, GUO-Meng, LIU-Xin, LI Chang-long, DU Xiao-yan, CHEN Zhen-wen, LV Jian-yi. Analysis on Cyclooxygenase 2 Expression in Different Tissues from Spontaneous Diabetic Mongolian Gerbils[J]. Laboratory Animal and Comparative Medicine, 2017, 37(2): 118-122.

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