Establishment of a Rat Model of Granulomatous Lobular Mastitis

doi:10.12300/j.issn.1674-5817.2020.149

Abstract

Abstract: Objective In this study, an animal model of granulomatous lobular mastitis was constructed using human granulomatous lobular mastitis specimen homogenates and a suspension of complete Freund's adjuvant. Methods Twenty-five rats with a history of maternity were randomly divided into five groups with five rats each: a blank control group, Freund's adjuvant group, normal mammary homogenate group, low-dose lesion homogenate group, and high-dose lesion homogenate group. In the lesion homogenization groups, rats were injected with complete Freund's adjuvant homogenate prepared from granulomatous mastitis patient tissues, and the third and fourth pair of mammary gland injections were administered to the rats at 0.2 mL in the high-dose group and 0.1 mL in the low-dose group. The normal mammary gland homogenate group was injected with normal mammary gland tissue homogenate from patients with granulomatous mastitis. The blank control group was injected with saline. The Freund's adjuvant group was injected with complete Freund's adjuvant. The histomorphological and pathophysiological characteristics of the mammary tissues were observed for 14 days after the completion of modeling, and the effects of the granulomatous lobular mastitis model were compared. Results The morphological changes and physiopathological characteristics of granulomatous lobular mastitis in the high- and low-dose groups were significantly different when compared with the blank control, Freund's adjuvant, and normal breast homogenate groups (P < 0.001). Conclusion Human granulomatous lobular mastitis specimen homogenates and suspensions of complete Freund's adjuvant for granulomatous lobular mastitis can successfully establish an animal model of the disease and lead to insights and new ideas for the etiology of the disease.

Key words: Granulomatous lobular mastitis, Animal model, SD rats

CLC Number:

Q95-33
R-332

ZUO Ximeng, SHI Xiaoguang, LIU Jieli, YANG Zhenrui, GAO Xiang, LAI Rui, ZHAO Ze, WANG Tangshun. Establishment of a Rat Model of Granulomatous Lobular Mastitis[J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 299-304.

Figures/Tables 3

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