常用近交系大鼠微卫星遗传位点筛选及分析

doi:10.12300/j.issn.1674-5817.2025.109

摘要/Abstract

摘要：

目的筛选一套覆盖大鼠1～20号常染色体和X染色体、每条染色体含2～4个短串联重复序列（short tandem repeat，STR，又称“微卫星”）位点的组合，并建立适用于5个常用近交系大鼠品系的遗传污染检测及品系鉴定的一套专用位点。方法取6～8周龄的F344、BN、DA、Lewis、PVG品系大鼠各6只（雌雄各半），抽提鼠尾组织基因组DNA。从国家标准GB 14923—2022和文献中分别遴选27和34个大鼠STR位点，共计61个。采用单重PCR扩增结合毛细管电泳技术对5个近交系大鼠品系样本进行分型，利用GeneMapper ID v3.2软件分析STR基因型数据。基于STR分型结果，通过GenAlEx 6.51b2软件计算品系间遗传距离，并借助MEGA7软件构建遗传进化树。结果在61个STR位点中，D15mit3存在非特异性扩增，D3wox7无特异性扩增产物，LCA、AGT、D5Hmgc2在5个品系间无多态性差异，其余56个STR位点具有品系间多态性，且覆盖大鼠1～20号常染色体和X染色体（每条染色体有2～4个STR位点）。其中42个STR位点可用于近交系大鼠遗传污染检测。D7wox14、D15rat123、D20wox3在5个品系中的扩增产物长度存在品系间差异，可用于品系鉴定。遗传进化树显示，BN与F344的STR位点等位基因差异数为49，遗传距离达1.775，二者遗传分化程度最高、亲缘关系最远；5个品系之间，F344、DA、PVG聚为一支，表明三者遗传距离相对较近；BN和Lewis品系聚为一支，表明二者遗传距离相对较近，5个品系的遗传亲缘关系与既往报道一致。结论本研究成功筛选到一套可用于F344、BN、DA、Lewis、PVG这5个常用近交系大鼠品系分子遗传检测的STR位点，并建立了分别用于遗传污染检测与品系间鉴定的专用位点组合。

关键词: 近交系大鼠, 微卫星位点, 分子遗传位点, 遗传进化树, 品系鉴定

Abstract:

Objective To screen a set of short tandem repeat (STR) markers covering rat chromosomes 1-20 and the X chromosome, with 2-4 markers on each chromosome, and establish a dedicated marker panel for genetic contamination detection and strain identification of 5 commonly used inbred rat strains. Methods Six 6-8 week-old rats (half male and half female) were selected from F344, BN, DA, Lewis, and PVG strain, respectively. Genomic DNA was extracted from rat tail tissues. A total of 61 STR markers were selected, including 27 from the national standard GB 14923—2022 and 34 from the literature. Singleplex STR PCR amplification combined with capillary electrophoresis was used for genotyping of the 5 inbred rat strains, and genotype data were analyzed using GeneMapper ID v3.2 software. Based on the STR genotyping results, genetic distances between strains were calculated with GenAlEx 6.51b2 software, and a phylogenetic tree of the inbred rat strains was constructed using MEGA7 software. Results Among the 61 STR markers, non-specific amplification was observed for D15mit3, while no specific amplification product was obtained for D3wox7. LCA, AGT, and D5Hmgc2 exhibited no inter-strain polymorphism across the 5 inbred strains, while the remaining 56 markers exhibited inter-strain polymorphism. These 56 markers covered rat chromosomes 1-20 and the X chromosome (2-4 markers per chromosome). Among them, 42 markers could be used for genetic contamination detection in inbred rat strains. The amplified product lengths of D7wox14, D15rat123, and D20wox3 differed among the 5 strains, which could be applied for strain identification. The phylogenetic tree showed that the number of STR allelic differences between BN and F344 strains was 49, with a genetic distance of 1.775, both of which were the highest among all strain pairs. This indicated that the two strains exhibited the highest level of genetic differentiation and were the most distantly related. Among the 5 strains, F344, DA, and PVG clustered together, indicating their relatively close genetic distance, while BN and Lewis formed another cluster, indicating a relatively close genetic distance between them. The genetic relationships among the 5 strains were consistent with previous reports. Conclusion This study successfully screened a set of STR markers for molecular genetic detection of 5 commonly used inbred rat strains, including F344, BN, DA, Lewis, and PVG, and established specific marker combinations for genetic contamination detection and strain identification, respectively.

Key words: Inbred rats, Microsatellite markers, Molecular genetic markers, Phylogenetic tree, Strain identification

中图分类号:

Q95-33

汤建平,赵丽亚,赵莹. 常用近交系大鼠微卫星遗传位点筛选及分析[J]. 实验动物与比较医学, 2026, 46(3): 388-396. DOI: 10.12300/j.issn.1674-5817.2025.109.

TANG Jianping,ZHAO Liya,ZHAO Ying. Screening and Analysis of Microsatellite Genetic Markers in Commonly Used Inbred Rat Strains[J]. Laboratory Animal and Comparative Medicine, 2026, 46(3): 388-396. DOI: 10.12300/j.issn.1674-5817.2025.109.

图/表 6

参考文献 23

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STR位点 STR marker	染色体 Chromosome	物理位置/bp^# Physical position/bp^#	正向引物序列（5'→3'） Upstream primer sequence（5'→3'）	反向引物序列（5'→3'） Downstream primer sequence（5'→3'）	退火温度/℃ Annealing temperature/℃
D1wox18	Chr1	100 133 276	TCACTCTCATTAAACTAGGAATGC	ACTGTTGGGTAACAAAGTTATGG	53
D1rat345^*	Chr1	112 693 166	TTCACACAAATGCCACCAGT	CAAAGAGATAGGGCGACAGG	59
D1wox21	Chr1	198 655 873	GACATGGTGAGTGAAGACCC	TGAGGCTATCCTGAGCTAACTC	59
D1mgh14^*	Chr1	278 228 767	CCGCACTGAGCTCTCAGAG	CCCAACCATTGAGCTAGTAAGG	61
D2rat3	Chr2	8 579 280	TCTCTGGTGGATACCTCACAAA	ACATGATGTGGAATTCCCCC	59
D2mgh26^*	Chr2	105 075 219	ATTGGTGATCAGATTTTTTTCTCC	CAGTTGAAAATTCCTGTATCTGG	57
D2wox15^*	Chr2	188 450 000	GGTGCTAGTAGACAATAAGATAGAT	TTCATGAGTTTTCACTGTTTGC	53
D2Arb20	Chr2	200 585 161	ACTAGAGTCAGGAGGCAAAACC	ACCCTATGTGAAATGGTGATGC	59
D3wox9^*	Chr3	51 821 710	TTCAGGTATGATTCGGGAAC	ATTGGCGATATCATTTCACTAAC	59
RH94469	Chr3	93 380 189	CTTATGTTACCTCACAGCCTGG	GGGTGGGCCATCTTTATAATC	61
D4wox27^*	Chr4	3 044 017	GGCCTTCATTTGTCCTTTGA	CACCATTATGCCCAGCCTTA	59
D4Arb10^*	Chr4	61 708 051	ACTCATCTTCTGAGGAGTAGCG	TTCACAGTCTATTTGGTAGGGC	59
D4mit15^*	Chr4	111 809 356	CCTAATGACAGCAATAGAAACTACG	TTTTGGTACTGGAGAAAGTTGG	61
D5mit18	Chr5	17 062 312	GCAAGACAGGCTTCCACAAT	AATACTGCCGGAGCAGAAAA	59
D5Hmgc2^*	Chr5	78 041 475	ATGTTCCCTGATGTCCCTTC	AAACCATGCCACGTGAAC	53
D5wox11	Chr5	138 180 823	CTTAGGTCAGCTGGTCCCTAG	CATGGTGCTTTTCCTTGTTG	55
D6rat105	Chr6	19 888 103	GCATGAGGCAGAGCCAATTA	GCCAGTTTGCCTTCACTTTC	59
D6mit1^*	Chr6	99 273 921	TTAGGAGAGAACTGAAAGTTGTCC	ATGGTGCACTATGGTGGTCA	61
D6wox12	Chr6	138 065 848	CTTCCCTACCTTCTACAACACTAAG	TAAACCAAAGAGCATGATGAAG	61
D7wox14	Chr7	102 588 256	GGGAGTAAAAGAGTGCATTCC	TACCCCAATCCTGAACCAC	61
D7wox24	Chr7	115 250 056	AGCCTCAATCTGGCCTAGCTC	TGACTCTTGACAATGGCACA	59
D7mgh3^*	Chr7	123 632 617	CGGCATGTGTCTCTGTGTG	TGACTTCTGGTGTCCTCACG	59
D8wox4^*	Chr8	50 529 563	GATTTGAAGCGATTGTCCAT	GTCTAGCTGCCCACAGGAG	57
D8rat14^*	Chr8	108 068 142	GCCCATACGTTGCATCAAGT	GGCCGGTCTAATTATTTCTTCA	59
D8wox11	Chr8	116 873 553	AGTGGGAATGCTTAGCCAAA	CAGGCCTTTACACCTTCCTG	57
D9mit2^*	Chr9	71 771 288	TGACCAGTTAGCCCTTTCCA	GGGAGCAGGGTTCTACACAG	59
D9rat110	Chr9	102 531 710	TGGGTGCTTAATTTTGTTTGTTT	GCTCCTAACCCATCCAACTTT	55
D10rat199	Chr10	1 381 054	TGCCAAATAATGATTGTTCCTTC	CCTCCTCTGGCTTCTTGAGA	55
D10wox12	Chr10	56 220 298	CGCTGGGAGGAAAGAGAG	AGGCTTGCACTCTGTGTTTG	59
D10mit12^*	Chr10	94 240 320	ATGGGAGGGAACAAGTCTTC	GAGGGAGAGAGAAAGAGAGACAG	61
D11wox3^*	Chr11	60 397 957	CACTTTGAGGCCATTCTGAA	CCTTCTCTTTGTGAAAAATAAAGTC	59
D11mgh3^*	Chr11	69 649 708	GGAGCTGAAATACGAGAGAAATAA	GTCCTGCTGGCTGTGCAT	59
D11wox2	Chr11	80 357 513	TTTCCAGGTGCCAAATGTAG	TATATTGTGACAAAAGAAAGGCAC	55
D12mit2^*	Chr12	22 650 702	GTGGCTCTTTTCCTTAGGGA	TCGGCTTCTGAATGTATTGG	55
D12rat36	Chr12	37 521 884	TGTAGAGAATCTAAAGCCCTCTTG	GAGGGACTTGGGAAGAGTGTT	61
D13rat60	Chr13	26 919 274	ACGGCACAATCAATTGATGA	AAACTCACATGCTTGCACGT	61
LCA^*	Chr13	52 147 717	TACAGAGCAAGCTCCAGGAC	TGTTTCTAATCCATAGGAAGTGC	59
D13rat131	Chr13	83 263 753	CAGAATCTTGTGCCTCCTGA	TATCGACTTGCCATTGTTCG	55
D14rat72	Chr14	8 058 678	CAGCAACACACACACCATACA	AGGAAGTCACACCACAAGGG	61
ALB^*	Chr14	19 191 541	TTTTCGTAGTAACGGAAGCC	TAAGGATTCTCAGATGCAAATG	59
D14wox14	Chr14	22 009 849	ACTTAATTACACACACAAACACAGA	CTTTGCTTTCTTTTAGCCATTT	61
D14rat39	Chr14	77 239 784	GGCTGATCCCCAAGTGTCTA	TGAATCAAAAGGGGCATTTC	59
D15rat55	Chr15	211 024	TATCCTCTTCTGCCCTCCAA	CCCACCCAGATGTGTTCTCT	59
D15rat123	Chr15	59 651 151	CCCCTACATCCATGGCATAC	TTCCCCCTCTTGTTCTTCCT	55
D16wox9^*	Chr16	18 740 885	ACCTACACGGACACATGGTG	GTTGTACTTCCTGATTTCCCTTT	61
D16rat15	Chr16	80 316 290	ATCGGTAAACAAATCGCTGG	TGGCATCTTTCGCTTAGACA	57
D17rat78	Chr17	42 541 872	TGCATGAGTCAATCTTCAATCA	CAGACTTCCTATTTTCCTATCTGCA	57
D17wox12^*	Chr17	63 994 211	AGGAAATTAAGAGAAGTTGGGACT	TATGCTCTTTGGGCAGCTTA	55
D18wox7	Chr18	15 539 551	GTGGAAAGCCTTCTGTTCAA	AGAATTCAATAATAACAGTCCCACT	61
TILP^*	Chr18	38 177 441	AATCACTGGATGCTGGAAGA	AGGGAGCAGAACTACTAAAGATACA	57
D18rat116	Chr18	40 057 808	TGCATATCCTTTTGCAACCA	TCCACTGTTCCAGGGAAGAC	55
D18rat8	Chr18	71 569 072	GCGGCTGGAGTTATGACTTT	GCCCACTCCTGTGTGAGATT	61
D19wox8	Chr19	24 455 726	TGCCCGTCTCTGTTACTCAT	CAAGAACCCTGAGGCAATAA	59
D19wox9	Chr19	42 099 630	GTACAGGAACACTGCCCTTG	GGAATGTGTATGTGTGATGACG	55
AGT^*	Chr19	69 426 540	TATGTAACTCAACGCCAGCC	GAAGCCCTAGTGGCAGATG	59
D20wox3^*	Chr20	4 855 475	AGGAAATGGGTTTCAGTTCC	CAGGATTCTGTGGCAATCCG	57
D20mit4	Chr20	36 923 763	TAAATTCAGAGTGTTGTTCCTGC	CACACCCAATGAATGCTCTG	59
DXwox6^*	ChrX	28 435 436	GTTTTCCCGCTTCACCAG	AGAAGGAGAAAGCGACCG	59
DXmgh9	ChrX	70 352 120	GTTGTCTGGTGCCAAAAGGT	AAGGATGATCTTAAGCTCCTGG	59

STR位点 STR marker	染色体 Chromosome	物理位置/bp^# Physical position/bp^#	正向引物序列（5'→3'） Upstream primer sequence（5'→3'）	反向引物序列（5'→3'） Downstream primer sequence（5'→3'）	退火温度/℃ Annealing temperature/℃
D1wox18	Chr1	100 133 276	TCACTCTCATTAAACTAGGAATGC	ACTGTTGGGTAACAAAGTTATGG	53
D1rat345^*	Chr1	112 693 166	TTCACACAAATGCCACCAGT	CAAAGAGATAGGGCGACAGG	59
D1wox21	Chr1	198 655 873	GACATGGTGAGTGAAGACCC	TGAGGCTATCCTGAGCTAACTC	59
D1mgh14^*	Chr1	278 228 767	CCGCACTGAGCTCTCAGAG	CCCAACCATTGAGCTAGTAAGG	61
D2rat3	Chr2	8 579 280	TCTCTGGTGGATACCTCACAAA	ACATGATGTGGAATTCCCCC	59
D2mgh26^*	Chr2	105 075 219	ATTGGTGATCAGATTTTTTTCTCC	CAGTTGAAAATTCCTGTATCTGG	57
D2wox15^*	Chr2	188 450 000	GGTGCTAGTAGACAATAAGATAGAT	TTCATGAGTTTTCACTGTTTGC	53
D2Arb20	Chr2	200 585 161	ACTAGAGTCAGGAGGCAAAACC	ACCCTATGTGAAATGGTGATGC	59
D3wox9^*	Chr3	51 821 710	TTCAGGTATGATTCGGGAAC	ATTGGCGATATCATTTCACTAAC	59
RH94469	Chr3	93 380 189	CTTATGTTACCTCACAGCCTGG	GGGTGGGCCATCTTTATAATC	61
D4wox27^*	Chr4	3 044 017	GGCCTTCATTTGTCCTTTGA	CACCATTATGCCCAGCCTTA	59
D4Arb10^*	Chr4	61 708 051	ACTCATCTTCTGAGGAGTAGCG	TTCACAGTCTATTTGGTAGGGC	59
D4mit15^*	Chr4	111 809 356	CCTAATGACAGCAATAGAAACTACG	TTTTGGTACTGGAGAAAGTTGG	61
D5mit18	Chr5	17 062 312	GCAAGACAGGCTTCCACAAT	AATACTGCCGGAGCAGAAAA	59
D5Hmgc2^*	Chr5	78 041 475	ATGTTCCCTGATGTCCCTTC	AAACCATGCCACGTGAAC	53
D5wox11	Chr5	138 180 823	CTTAGGTCAGCTGGTCCCTAG	CATGGTGCTTTTCCTTGTTG	55
D6rat105	Chr6	19 888 103	GCATGAGGCAGAGCCAATTA	GCCAGTTTGCCTTCACTTTC	59
D6mit1^*	Chr6	99 273 921	TTAGGAGAGAACTGAAAGTTGTCC	ATGGTGCACTATGGTGGTCA	61
D6wox12	Chr6	138 065 848	CTTCCCTACCTTCTACAACACTAAG	TAAACCAAAGAGCATGATGAAG	61
D7wox14	Chr7	102 588 256	GGGAGTAAAAGAGTGCATTCC	TACCCCAATCCTGAACCAC	61
D7wox24	Chr7	115 250 056	AGCCTCAATCTGGCCTAGCTC	TGACTCTTGACAATGGCACA	59
D7mgh3^*	Chr7	123 632 617	CGGCATGTGTCTCTGTGTG	TGACTTCTGGTGTCCTCACG	59
D8wox4^*	Chr8	50 529 563	GATTTGAAGCGATTGTCCAT	GTCTAGCTGCCCACAGGAG	57
D8rat14^*	Chr8	108 068 142	GCCCATACGTTGCATCAAGT	GGCCGGTCTAATTATTTCTTCA	59
D8wox11	Chr8	116 873 553	AGTGGGAATGCTTAGCCAAA	CAGGCCTTTACACCTTCCTG	57
D9mit2^*	Chr9	71 771 288	TGACCAGTTAGCCCTTTCCA	GGGAGCAGGGTTCTACACAG	59
D9rat110	Chr9	102 531 710	TGGGTGCTTAATTTTGTTTGTTT	GCTCCTAACCCATCCAACTTT	55
D10rat199	Chr10	1 381 054	TGCCAAATAATGATTGTTCCTTC	CCTCCTCTGGCTTCTTGAGA	55
D10wox12	Chr10	56 220 298	CGCTGGGAGGAAAGAGAG	AGGCTTGCACTCTGTGTTTG	59
D10mit12^*	Chr10	94 240 320	ATGGGAGGGAACAAGTCTTC	GAGGGAGAGAGAAAGAGAGACAG	61
D11wox3^*	Chr11	60 397 957	CACTTTGAGGCCATTCTGAA	CCTTCTCTTTGTGAAAAATAAAGTC	59
D11mgh3^*	Chr11	69 649 708	GGAGCTGAAATACGAGAGAAATAA	GTCCTGCTGGCTGTGCAT	59
D11wox2	Chr11	80 357 513	TTTCCAGGTGCCAAATGTAG	TATATTGTGACAAAAGAAAGGCAC	55
D12mit2^*	Chr12	22 650 702	GTGGCTCTTTTCCTTAGGGA	TCGGCTTCTGAATGTATTGG	55
D12rat36	Chr12	37 521 884	TGTAGAGAATCTAAAGCCCTCTTG	GAGGGACTTGGGAAGAGTGTT	61
D13rat60	Chr13	26 919 274	ACGGCACAATCAATTGATGA	AAACTCACATGCTTGCACGT	61
LCA^*	Chr13	52 147 717	TACAGAGCAAGCTCCAGGAC	TGTTTCTAATCCATAGGAAGTGC	59
D13rat131	Chr13	83 263 753	CAGAATCTTGTGCCTCCTGA	TATCGACTTGCCATTGTTCG	55
D14rat72	Chr14	8 058 678	CAGCAACACACACACCATACA	AGGAAGTCACACCACAAGGG	61
ALB^*	Chr14	19 191 541	TTTTCGTAGTAACGGAAGCC	TAAGGATTCTCAGATGCAAATG	59
D14wox14	Chr14	22 009 849	ACTTAATTACACACACAAACACAGA	CTTTGCTTTCTTTTAGCCATTT	61
D14rat39	Chr14	77 239 784	GGCTGATCCCCAAGTGTCTA	TGAATCAAAAGGGGCATTTC	59
D15rat55	Chr15	211 024	TATCCTCTTCTGCCCTCCAA	CCCACCCAGATGTGTTCTCT	59
D15rat123	Chr15	59 651 151	CCCCTACATCCATGGCATAC	TTCCCCCTCTTGTTCTTCCT	55
D16wox9^*	Chr16	18 740 885	ACCTACACGGACACATGGTG	GTTGTACTTCCTGATTTCCCTTT	61
D16rat15	Chr16	80 316 290	ATCGGTAAACAAATCGCTGG	TGGCATCTTTCGCTTAGACA	57
D17rat78	Chr17	42 541 872	TGCATGAGTCAATCTTCAATCA	CAGACTTCCTATTTTCCTATCTGCA	57
D17wox12^*	Chr17	63 994 211	AGGAAATTAAGAGAAGTTGGGACT	TATGCTCTTTGGGCAGCTTA	55
D18wox7	Chr18	15 539 551	GTGGAAAGCCTTCTGTTCAA	AGAATTCAATAATAACAGTCCCACT	61
TILP^*	Chr18	38 177 441	AATCACTGGATGCTGGAAGA	AGGGAGCAGAACTACTAAAGATACA	57
D18rat116	Chr18	40 057 808	TGCATATCCTTTTGCAACCA	TCCACTGTTCCAGGGAAGAC	55
D18rat8	Chr18	71 569 072	GCGGCTGGAGTTATGACTTT	GCCCACTCCTGTGTGAGATT	61
D19wox8	Chr19	24 455 726	TGCCCGTCTCTGTTACTCAT	CAAGAACCCTGAGGCAATAA	59
D19wox9	Chr19	42 099 630	GTACAGGAACACTGCCCTTG	GGAATGTGTATGTGTGATGACG	55
AGT^*	Chr19	69 426 540	TATGTAACTCAACGCCAGCC	GAAGCCCTAGTGGCAGATG	59
D20wox3^*	Chr20	4 855 475	AGGAAATGGGTTTCAGTTCC	CAGGATTCTGTGGCAATCCG	57
D20mit4	Chr20	36 923 763	TAAATTCAGAGTGTTGTTCCTGC	CACACCCAATGAATGCTCTG	59
DXwox6^*	ChrX	28 435 436	GTTTTCCCGCTTCACCAG	AGAAGGAGAAAGCGACCG	59
DXmgh9	ChrX	70 352 120	GTTGTCTGGTGCCAAAAGGT	AAGGATGATCTTAAGCTCCTGG	59

STR位点 STR marker	各品系的产物长度/bp Product size of each strain/bp					等位基因数 Number of alleles	STR位点 STR marker	各品系的产物长度/bp Product size of each strain/bp					等位基因数 Number of alleles
STR位点 STR marker	F344	BN	DA	Lewis	PVG	等位基因数 Number of alleles	STR位点 STR marker	F344	BN	DA	Lewis	PVG	等位基因数 Number of alleles
D1wox18	122	120	122	120	118	3	D11wox3^*	189	183	183	185	181	4
D1rat345^*	125	125	121	125	129	3	D11mgh3^*	150	166	186	145	145	4
D1wox21	85	97	73	95	97	4	D11wox2	152	146	148	146	148	3
D1mgh14^*	142	120	150	150	140	4	D12mit2^*	187	215	187	206	196	4
D2rat3	126	122	124	117	122	4	D12rat36	205	202	205	188	190	4
D2mgh26^*	126	132	134	134	126	3	D13rat60	131	125	125	127	127	3
D2wox15^*	151	131	133	133	145	4	D13rat131	149	151	149	134	134	3
D2Arb20	231	235	207	215	207	4	D14rat72	152	181	162	179	152	4
D3wox9^*	117	123	115	121	121	4	ALB^*	136	120	136	133	136	3
RH94469	239	239	239	227	221	3	D14wox14	122	112	122	108	108	3
D4wox27^*	214	197	219	197	214	3	D14rat39	240	248	240	248	240	2
D4Arb10^*	389	392	389	389	409	3	D15rat55	210	228	228	228	228	2
D4mit15^*	215	215	215	215	202	2	D15rat123	136	132	130	122	120	5
D5mit18	235	219	225	235	231	4	D16wox9^*	145	137	191	145	145	3
D5wox11	137	123	137	137	137	2	D16rat15	142	133	144	133	144	3
D6rat105	229	225	204	227	227	4	D17rat78	151	157	155	155	155	3
D6mit1^*	134	122	112	134	134	3	D17wox12^*	136	132	115	119	115	4
D6wox12	127	127	135	127	127	2	D18wox7	148	142	189	193	189	4
D7wox14	120	126	122	118	124	5	TILP^*	262	240	262	242	242	3
D7wox24	133	133	133	133	129	2	D18rat116	219	223	219	223	225	3
D7mgh3^*	233	243	233	243	233	2	D18rat8	155	165	157	165	161	4
D8wox4^*	131	119	129	131	117	4	D19wox8	124	126	111	124	111	3
D8rat14^*	169	161	161	161	169	2	D19wox9	154	158	158	156	160	4
D8wox11	183	191	181	191	183	3	D20wox3^*	116	110	120	128	114	5
D9mit2^*	185	185	190	190	185	2	D20mit4	215	201	192	198	215	4
D9rat110	146	158	169	158	146	3	Dxwox6^*	171	138	173	138	175	4
D10rat199	190	188	188	188	188	2	Dxmgh9	120	146	146	146	120	2
D10wox12	95	95	95	91	91	2	纯合度 Homozygosity	100%	100%	100%	100%	100%	-
D10mit12^*	142	144	144	144	144	2	纯合度 Homozygosity	100%	100%	100%	100%	100%	-

STR位点 STR marker	各品系的产物长度/bp Product size of each strain/bp					等位基因数 Number of alleles	STR位点 STR marker	各品系的产物长度/bp Product size of each strain/bp					等位基因数 Number of alleles
STR位点 STR marker	F344	BN	DA	Lewis	PVG	等位基因数 Number of alleles	STR位点 STR marker	F344	BN	DA	Lewis	PVG	等位基因数 Number of alleles
D1wox18	122	120	122	120	118	3	D11wox3^*	189	183	183	185	181	4
D1rat345^*	125	125	121	125	129	3	D11mgh3^*	150	166	186	145	145	4
D1wox21	85	97	73	95	97	4	D11wox2	152	146	148	146	148	3
D1mgh14^*	142	120	150	150	140	4	D12mit2^*	187	215	187	206	196	4
D2rat3	126	122	124	117	122	4	D12rat36	205	202	205	188	190	4
D2mgh26^*	126	132	134	134	126	3	D13rat60	131	125	125	127	127	3
D2wox15^*	151	131	133	133	145	4	D13rat131	149	151	149	134	134	3
D2Arb20	231	235	207	215	207	4	D14rat72	152	181	162	179	152	4
D3wox9^*	117	123	115	121	121	4	ALB^*	136	120	136	133	136	3
RH94469	239	239	239	227	221	3	D14wox14	122	112	122	108	108	3
D4wox27^*	214	197	219	197	214	3	D14rat39	240	248	240	248	240	2
D4Arb10^*	389	392	389	389	409	3	D15rat55	210	228	228	228	228	2
D4mit15^*	215	215	215	215	202	2	D15rat123	136	132	130	122	120	5
D5mit18	235	219	225	235	231	4	D16wox9^*	145	137	191	145	145	3
D5wox11	137	123	137	137	137	2	D16rat15	142	133	144	133	144	3
D6rat105	229	225	204	227	227	4	D17rat78	151	157	155	155	155	3
D6mit1^*	134	122	112	134	134	3	D17wox12^*	136	132	115	119	115	4
D6wox12	127	127	135	127	127	2	D18wox7	148	142	189	193	189	4
D7wox14	120	126	122	118	124	5	TILP^*	262	240	262	242	242	3
D7wox24	133	133	133	133	129	2	D18rat116	219	223	219	223	225	3
D7mgh3^*	233	243	233	243	233	2	D18rat8	155	165	157	165	161	4
D8wox4^*	131	119	129	131	117	4	D19wox8	124	126	111	124	111	3
D8rat14^*	169	161	161	161	169	2	D19wox9	154	158	158	156	160	4
D8wox11	183	191	181	191	183	3	D20wox3^*	116	110	120	128	114	5
D9mit2^*	185	185	190	190	185	2	D20mit4	215	201	192	198	215	4
D9rat110	146	158	169	158	146	3	Dxwox6^*	171	138	173	138	175	4
D10rat199	190	188	188	188	188	2	Dxmgh9	120	146	146	146	120	2
D10wox12	95	95	95	91	91	2	纯合度 Homozygosity	100%	100%	100%	100%	100%	-
D10mit12^*	142	144	144	144	144	2	纯合度 Homozygosity	100%	100%	100%	100%	100%	-

染色体 Chromosome	STR位点 STR marker
Chr1	D1wox18^#、D1wox21^&、D1mgh14^&
Chr2	D2rat3^&、D2mgh26^#、D2Arb20^&
Chr3	D3wox9^&、RH94469^#
Chr4	D4wox27^#、D4Arb10^#
Chr5	D5mit18^&、D5wox11^∗
Chr6	D6rat105^&、D6mit1^#
Chr7	D7wox14^†、D7mgh3^∗
Chr8	D8wox4^&、D8wox11^#
Chr9	D9mit2^∗、D9rat110^#
Chr10	D10rat199^∗、D10mit12^∗
Chr11	D11wox3^&、D11wox2^#
Chr12	D12mit2^&、D12rat36^&
Chr13	D13rat60^#、D13rat131^#
Chr14	D14rat72^&、D14wox14^#
Chr15	D15rat55^∗、D15rat123^†
Chr16	D16wox9^#、D16rat15^#
Chr17	D17rat78^#、D17wox12^&
Chr18	D18wox7^&、D18rat8^&
Chr19	D19wox8^#、D19wox9^&
Chr20	D20wox3^†、D20mit4^&