槲皮素对镉致小鼠肝脏脂质代谢紊乱模型的保护效应

doi:10.12300/j.issn.1674-5817.2021.007

摘要/Abstract

摘要： 目的探究槲皮素对镉致小鼠肝损伤的保护效应及机制。方法 C57BL/6J小鼠分为对照组、镉组（灌胃氯化镉）、槲皮素组（灌胃槲皮素）、镉与槲皮素共处理组（灌胃氯化镉+槲皮素）。给药结束后,测定小鼠体质量、血清中谷草转氨酶（AST）和谷丙转氨酶（ALT）活性及血清与肝组织中总胆固醇（TC）和三酰甘油（TG）含量;HE染色观察肝组织病理学变化,油红O染色观察肝组织脂滴变化;免疫印迹法检测脂质合成关键酶脂肪酸合成酶（FAS）与分解关键酶脂蛋白脂酶（LPL）变化。结果与对照组相比,镉染毒小鼠体质量显著下降（P<0.01）,血清ALT与AST活性显著升高（P<0.01）,肝组织发生明显肝损伤病理学变化,血清与肝脏中TC和TG含量明显上升（P<0.01）,且肝组织内脂肪滴大量沉积（P<0.01）,肝脏中FAS蛋白水平显著上调（P<0.01）,而LPL蛋白水平显著下调（P<0.01）。槲皮素干预可显著改善以上各项指标的异常变化（P<0.05或P<0.01）。结论槲皮素可通过调节肝脏脂质代谢紊乱,对镉导致的小鼠肝损伤起到保护效应。

关键词: 槲皮素, 镉, 肝脏损伤, 脂质代谢, C57BL/6J小鼠

Abstract: Objective To investigate the protective effect of quercetin on cadmium-mediated lipid metabolism disorder in livers of mice. Methods C57BL/6J mice were divided into four groups: a control group, a quercetin-treated group (quercetin by intragastrical administration), a cadmium-treated group (cadmium chloride by intragastrical administration), and a cadmium plus quercetin co-treated group (cadmium chloride plus quercetin by intragastrical administration). After the administration, mice weight was measured; the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, and the contents of total cholesterol (TC) and triglyceride (TG) in both serum and liver were determined; the pathological changes in liver tissues and liver droplets were observed by HE and Oil Red O staining; the expression levels of proteins related to lipogenesis, fatty acid synthase (FAS), and related to lipolysis, lipoprotein lipase (LPL), in liver were detected by Western blotting. Results Compared with the control group, the mice treated with cadmium showed that the weight was significantly decreased (P<0.01), the ALT and AST activities were remarkedly increased in serum (P<0.01), the pathological changes of liver injury were seen obviously, the TC and TG contents were remarkedly elevated in both serum and liver (P<0.01), lipid droplets were greatly accumulated (P<0.01), the FAS expression in liver was up-regulated (P<0.01), while the LPL expression was down-regulated (P<0.01). Quercetin intervention significantly alleviated the abnormal changes of the above indicators. Conclusion Quercetin can protect mice from cadmium-induced liver injury by regulating lipid metabolism disorder in liver.

Key words: Quercetin, Cadmium, Liver damage, Lipid metabolism, C57BL/6J mice

中图分类号:

Q95-33

李自发, 张浩, 任萌, 徐凯勇, 胡明会, 周苗苗, 王可洲. 槲皮素对镉致小鼠肝脏脂质代谢紊乱模型的保护效应[J]. 实验动物与比较医学, 2021, 41(4): 305-312.

LI Zifa, ZHANG Hao, REN Meng, XU Kaiyong, HU Minghui, ZHOU Miaomiao, WANG Kezhou. Protective Effect of Quercetin on Lipid Metabolism Disorder in Mice Livers Caused by Cadmium[J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 305-312.

图/表 5

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