还原型谷胱甘肽对C57BL/6J小鼠体外受精的影响

doi:10.3969/j.issn.1674-5817.2016.03.010

摘要/Abstract

摘要： 目的探讨受精液中添加还原型谷胱甘肽(GSH)对C57BL/6J小鼠体外受精的影响。方法以体外受精液(HTF)作为对照, 在HTF液中添加不同浓度(0.25 mmol/L、0.50 mmol/L、0.75 mmol/L、1.00 mmol/L、1.25 mmol/L和1.50 mmol/L)的GSH,对C57BL/6J近交系小鼠进行体外受精实验,观察体外受精和早期胚胎发育情况。同时,用5种C57BL/6J小鼠背景的基因工程小鼠加以验证。结果与对照组相比,实验组的体外受精率显著升高,浓度为0.50 mmol/L时,体外受精率提高显著(P<0.05),浓度在1.25 mmol/L时,体外受精率最高(P<0.01)。受精卵的体外囊胚发育率实验组与对照组无显著性差异(P>0.05)。C57BL/6J背景的基因工程小鼠的体外受精率和体外囊胚发育率与C57BL/6J近交系小鼠相似。结论在HTF中添加一定浓度的GSH能够提高C57BL/6J小鼠及C57BL/6J背景的基因工程小鼠的体外受精效果。

关键词: 还原型谷胱甘肽(GSH), C57BL/6J小鼠, 体外受精

Abstract: Objective To study the influence of reduced glutathione (GSH) in fertilization medium on the in vitro fertilization (IVF) in C57BL/6J mouse. Methods The fertilization medium without reduced glutathione was prepared as control group, and the different concentrations of GSH (0.25 mmol/L, 0.50 mmol/L, 0.75mmol/L, 1.00mmol/L, 1.25mmol/L and 1.50mmol/L) were added to the fertilization medium during IVF, which were divided into 6 experimental groups, to investigate its effect on the fertilization and early embryo development of C57BL/6J inbred mice. In addition, 5 strains of genetically engineered mice having a genetic background of C57BL/6J were chosen to verify the influence of GSH. Results Compared with the control, GSH increased the fertilization rate in a dose-dependent manner. Statistically significant increased fertilization was first observed at 0.5 mmol/L GSH (P<0.05), reaching maximum values at 1.25 mmol/L(P<0.01). The blastocyst developmental rates have no significant difference from that of the control group (P>0.05). Fertilization and development rates of the genetically engineered mice were similar to those of C57BL/6 inbred mice. Conclusion In a certain range of concentration of GSH during the IVF procedure, it can enhance the fertility of C57BL/6J mouse and the genetically engineered mice having a genetic background of C57BL/6J.

Key words: Reduced glutathione(GSH), C57BL/6J mouse, In vitro fertilization (IVF)

中图分类号:

Q95-33

赵晨, 张双琴, 王利凤, 李薇. 还原型谷胱甘肽对C57BL/6J小鼠体外受精的影响[J]. 实验动物与比较医学, 2016, 36(3): 208-211.

ZHAO Chen, ZHANG Shuang-qin, Wang Li-feng, LI Wei. Influence of Reduced Glutathione Added Medium on in vitro Fertilization in C57BL/6J Mouse[J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 208-211.

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