异功散对小鼠慢性病贫血的治疗作用

doi:10.3969/j.issn.1674-5817.2017.06.001

实验动物与比较医学 ›› 2017, Vol. 37 ›› Issue (6): 421-427.DOI: 10.3969/j.issn.1674-5817.2017.06.001

• 论著 • 下一篇

异功散对小鼠慢性病贫血的治疗作用

郑秦¹, 姜一陵¹, 季玉婷¹, 夏乐敏¹, 张爱萍¹, 石岭¹, 戴程怡¹, 薛城¹, 邹纯朴², 罗梅宏¹

1.上海市宝山区中西医结合医院暨上海中医药大学附属曙光医院宝山分院血液科, 上海201999;
2.上海中医药大学基础医学院内经教研室, 上海 201203

收稿日期:2017-08-10 出版日期:2017-12-25 发布日期:2017-12-25
作者简介:郑秦(1986-),硕士研究生,住院医师,主要研究方向:中医药治疗血液病的临床与实验研究。E-mail:670251899@qq.com
基金资助:
国家自然科学基金青年项目(81102569),国家自然基金面上项目(81774271),上海卫生和计划生育委员会科研项目(201440587),上海市自然科学基金面上项目(16ZR1425400)

Therapeutic Effects of Yi Gong San Decoction on Anemia of Chronic Disease in Mouse

ZHENG Qin¹, JIANG Yi-ling¹, JI Yuting¹, XIA Le-ming¹, ZHANG Ai-ping¹, SHI Ling¹, DAI Cheng-yi¹, XUE Cheng¹, ZOU Chun-pu², LUO Mei-hong¹

1. Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine Baoshan Branch of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201900, China;
2. Neijing Department, School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Received:2017-08-10 Online:2017-12-25 Published:2017-12-25

摘要/Abstract

摘要： 目的观察异功散(YGS)对慢性病贫血(ACD)小鼠的治疗作用。方法取8周龄雄性C57BL/6小鼠,腹腔注射脂多糖(0.5 mg/mL), 第6 d腹腔注射酵母糖A(32 mg/mL),第11 d模型小鼠随机分为模型组、中药干预组,另设空白对照组。当日起给予药物干预,中药干预组灌胃YGS水煎液,剂量为每日15.413 g/kg生药; 空白对照组、模型组灌胃0.2 mL超纯水。采集外周血、肝、脾组织, 采用自动细胞计数仪检测小鼠血常规; 比色法检测血清铁(SI)、未饱和铁结合力(UIBC); ELISA法检测铁蛋白(SF); 普鲁士蓝染色观察肝、脾组织铁,采用Image-Pro Plus 6.0软件分析普鲁士蓝铁离子阳性面积百分比,RT-PCR检测小鼠肝脏铁调素基因(HAMP) mRNA的表达。结果与模型组比,连续给药1周和2周后中药干预组小鼠血红蛋白(HB)、红细胞计数(RBC)、红细胞比容(HCT)均有显著提高(P<0.01或P<0.05),红细胞压积(MCV)无明显改善(P>0.05), 血清SI和UIBC也明显上升(P<0.01或P<0.05), 血清SF及肝、脾组织铁含量显著降低(P<0.01或P<0.05), 肝脏HAMP mRNA显著下降(P<0.01或P<0.05)。结论 YGS可以改善ACD小鼠贫血,调节其铁代谢的紊乱,这一作用可能与其对铁调素的下调有关。

关键词: 慢性病贫血(ACD), 异功散(YGS), 治疗, 铁调素

Abstract: Objective To observe the therapeutic effects of Yi Gong San decoction (YGS) on anemia of chronic disease (ACD) in mouse. Methods Totally 48 male 8-week-old male C57 BL/6 mice were randomly divided into blank control group, model group, intervention group. Each group was sacrificed 8 mouse after the continuous administration of 1 week or 2 weeks. The model group and intervention group intraperitoneally injected with zymosan A after 6 days of intraperitoneally injected with LPS. After 5 days of zymosan A injection, YGS (15.413 g per day) was administered to mice intragastrically in intervention group for successive 1 or 2 weeks while 0.2 mL of ultra-pure water was administered to mice intragastrically in blank control group and the model group. The mouse blood routine was measured by automatic cell counting. Colorimetric method was used to detect serum iron (SI) and unsaturated iron binding (UIBC). ELISA was used to detect ferritin (SF). The ratio of iron in liver and spleen were analyzed by software using Prussian blue staining. The positive area of Prussian blue iron was analyzed by Image-Pro Plus 6.0 software. The expression of hepcidin antimicrobial peptide (HAMP) mRNA in mouse liver was detected by RT-PCR. Results In the 1 or 2 weeks, the content of hemoglobin (HB), erythrocyte count (RBC), hematocrit (HCT) in mice was significantly increased (vs model group, P<0.01 or P<0.05); Hematocrit (MCV) was not changed (P>0.05). The SI and UIBC was increased (VS model group, P<0.01 or P<0.05); the SF and the ratio of iron in liver and spleen reduced significantly (VS model group, P<0.01or P<0.05). Liver HAMP mRNA was significantly decreased (P<0.01 or P<0.05). Conclusion Yi Gong San decoction can improve the degree of anemia and adjust the iron metabolism disorder in mouse of anemia of chronic disease by decreasing HAMP mRNA relative expression.

Key words: Anemia of chronic disease (ACD), Yi Gong San decoction (YGS), Treatment, Hepcidin

中图分类号:

Q95-33

郑秦, 姜一陵, 季玉婷, 夏乐敏, 张爱萍, 石岭, 戴程怡, 薛城, 邹纯朴, 罗梅宏. 异功散对小鼠慢性病贫血的治疗作用[J]. 实验动物与比较医学, 2017, 37(6): 421-427.

ZHENG Qin, JIANG Yi-ling, JI Yuting, XIA Le-ming, ZHANG Ai-ping, SHI Ling, DAI Cheng-yi, XUE Cheng, ZOU Chun-pu, LUO Mei-hong. Therapeutic Effects of Yi Gong San Decoction on Anemia of Chronic Disease in Mouse[J]. Laboratory Animal and Comparative Medicine, 2017, 37(6): 421-427.

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异功散对小鼠慢性病贫血的治疗作用

Therapeutic Effects of Yi Gong San Decoction on Anemia of Chronic Disease in Mouse

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