实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (2): 99-104.DOI: 10.3969/j.issn.1674-5817.2019.02.006

• 论著 • 上一篇    下一篇

胰岛素抵抗合并高尿酸血症大鼠模型的建立及其比较

刘孟渊1, 冯学轩2, 黎莉斯2, 张美艺1   

  1. 1.广州市中医医院, 广州 510130;
    2.广东省医学实验动物中心, 佛山 528248
  • 收稿日期:2018-07-02 出版日期:2019-04-25 发布日期:2021-01-29
  • 作者简介:刘孟渊(1964-),硕士,教授,从事内科疾病的中西医结合临床及科研工作。E-mail:mymyliu@sina.cn
  • 基金资助:
    广东省中管局建设中医药强省立项资助科研课题 (20151066), 广州市医药卫生科技项目(20152A011004), 广州市科技创新委员会科学(技术)研究专项一般项目(201607010303)

Establishment and Comparison of Rat Models of Insulin Resistance Combined with Hyperuricemia

LIU Meng-yuan1, FENG Xue-xuan2, LI Li-si2, ZHANG Mei-yi1   

  1. 1. Guangzhou Hospital of TCM, Guangzhou 510130, China;
    2. Guangdong Medical Laboratory Animal Center, Foshan 528248, China
  • Received:2018-07-02 Online:2019-04-25 Published:2021-01-29

摘要: 目的 为制备成功、稳定的胰岛素抵抗合并高尿酸血症(IR-HUA)模型,比较了IR-HUA 模型、高尿酸血症(HUA) 模型、胰岛素抵抗(IR)模型的制备方法。方法 选用40只雄性SD大鼠,按体质量随机分为正常对照组(A组)、HUA模型组(B组)、IR-HUA模型组(C组)和IR模型组(D组), A组、B组喂饲普通饲料及饮用水; C组、D组喂饲自制高脂饲料及质量分数10%果糖水; 造模8周, 第6周始, B组、C组大鼠每日灌胃酵母膏(16 g/kg) 21 d; 于实验第6、8周末取样检测相关指标。结果 实验第8周末, 与A组相比, B组血尿酸(SUA)、尿尿酸(UUA)、尿酸排泄总量及尿酸排泄分数(FEUA)均升高; C组空腹胰岛素(FINS)、稳态模型胰岛素抵抗指数(HOMA-IR)值均下降,UUA浓度、FEUA均升高, 排尿体积下降; D组Lee’s指数下降, 空腹血糖(FPG)升高, FINS下降, UUA浓度升高, 排尿体积、尿酸排泄总量均下降, 血、尿肌酐均升高。结论 给雄性SD大鼠每日灌胃酵母膏(16 g/kg) 21 d可制备HUA模型; 单纯给予高脂高糖饮食8周诱导大鼠IR模型欠理想, 大鼠未呈现出明显的IR特征, IR-HUA模型制备方法有待进一步完善。此外, 高脂高糖饮食可致大鼠排尿体积减少, 尿酸排泄下降; 并可致大鼠胰岛素分泌减少, 导致血糖升高。

关键词: 胰岛素抵抗合并高尿酸血症(IR-HUA), 空腹血糖(FPG), 动物模型

Abstract: Objective To prepare successful, stable and optimized insulin resistance combined with hyperuricemia (IR-HUA) models, the preparation methods of IR-HUA model, HUA model and IR model in rats were compared. Method Totally 40 male SD rats were randomly divided into the normal control group (A), the HUA model group (B), the IR-HUA model group (C), and the IR model group (D). The group A and group B were fed with the ordinary feed and drinking water, the group C and group D were fed with the high fat feed and high sugar diet and drinking 10% fructose water. During 8 weeks for modeling, at sixth weeks, rats in group B and group C were fed with yeast extract (16 g/kg/per day) 21d, At the end of sixth, eighth week, the observation index were detected. Result At the eighth weekend of the experiment, compared with group A, the concentrations of serum uric acid (SUA) and urine uric acid (UUA), total uric acid excretion and the fraction excretion of uric acid (FEUA) in group B increased; The levels of fasting insulin (FINS) and HOMA-IR index, and urination volume in group C decreased; the concentration of UUA and FEUA in group C increased; the levels of Lee 's index, FINS, urination volume,and total uric acid excretion in group D declined, the concentrations of fasting plasma glucose(FPG), UUA, serum creatinine (SCr) and uric creatinine (UCr) in group D increased. Conclusion HUA model could be prepared in SD rats by fed with yeast extract 16 g/kg/per day for 21d. The IR model in SD rats induced by high fat and high sugar diet for 8 weeks was not ideal, it could be due to the shorter molding time. The rat model showed no obvious characteristics of IR. The preparation method of IR-HUA model needs to be further improved. In addition, high-fat and high-sugar diet can reduce the volume of urine and the excretion of uric acid in rats, and reduce the secretion of insulin in rats, leading to the increase of FPG.

Key words: Insulin resistance combined with Hyperuricemia (IR-HUA), Fasting plasma glucose (FPG), Animal model

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