肿瘤类器官模型的建立及应用

doi:10.3969/j.issn.1674-5817.2020.06.015

实验动物与比较医学 ›› 2020, Vol. 40 ›› Issue (6): 540-546.DOI: 10.3969/j.issn.1674-5817.2020.06.015

肿瘤类器官模型的建立及应用

罗宝花^1,2*, 张永斌^1*, 刘晓秋^1#, 师长宏^2#

1.广州中医药大学科技创新中心/实验动物中心,广州 510405;
2.空军军医大学实验动物中心,西安 710032

收稿日期:2020-04-20 出版日期:2020-12-25 发布日期:2020-12-30
作者简介:罗宝花(1995-), 女, 硕士研究生, 研究方向:中医肿瘤学。 E-mail: 2412190275@qq.com张永斌(1973-), 男, 副研究员, 研究方向: 动物模型研究。E-mail: yongbinzhang@gzucm.edu.cn
^*共同第一作者
基金资助:
国家自然科学基金(31772546)

Establishment and Applications of Tumor Organoid Models

LUO Baohua^1,2*, ZHANG Yongbin^1*, LIU Xiaoqiu^1#, SHI Changhong^2#

1. Innovation Centre for Science and Technology/Laboratory Animal Center,Guangzhou University of Chinese Medicine, Guangzhou 510405, China;
2. Laboratory Animal Center, Air Force Medical University, Xi'an 710032, China

Received:2020-04-20 Online:2020-12-25 Published:2020-12-30

摘要/Abstract

摘要： 来源于特异性干细胞,经体外三维培养,形成与原器官相类似的空间形态结构,称为类器官（organoid）。运用该培养技术形成的肿瘤类器官,可以较好地再现原发肿瘤的体内特征及异质性,而且所需形成时间短、传代稳定,弥补了传统肿瘤模型的缺陷,可用于肿瘤的发生和发展机制研究、药物筛选与个体化治疗、免疫治疗和液体活检等。本文将从肿瘤类器官模型的发展简史、建立方法、应用前景及局限性等方面进行综述。

关键词: 类器官, 肿瘤研究, 三维培养, 个体化治疗

Abstract: The organoid is derived from specific stem cells for in vitro three-dimensional culture to form a spatial structure, which keeps similar biological characteristics to the original organ. By this culture technology, the tumor organoids can be established. It well retains the feature in vivo and heterogeneity of the original tumor tissues, displays the advantages of short culture cycle and stable genomes after multiple passages, which compensates the defects of traditional tumor models. These tumor organoids can be used to study the mechanism of tumor development, drug screening and individualized treatment, immunotherapy, liquid biopsy, etc. In this paper, the development history, establishment methods, application prospects and limitations of tumor organoid models are reviewed.

Key words: Organoid, Tumor research, Three-dimensional culture, Individualized treatment

中图分类号:

Q95-33
R73-3

罗宝花, 张永斌, 刘晓秋, 师长宏. 肿瘤类器官模型的建立及应用[J]. 实验动物与比较医学, 2020, 40(6): 540-546.

LUO Baohua, ZHANG Yongbin, LIU Xiaoqiu, SHI Changhong. Establishment and Applications of Tumor Organoid Models[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 540-546.

图/表 3

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肿瘤类器官模型的建立及应用

Establishment and Applications of Tumor Organoid Models

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