四种群东方田鼠线粒体DNA

›› 2008, Vol. 28 ›› Issue (5): 299-303.

所属专题：实验动物资源开发与利用

四种群东方田鼠线粒体DNA

1.上海实验动物研究中心，上海实验动物监督检验站，上海 200032；2.上海计划生育科学研究所，上海 200032；3.上海西普尔-必凯实验动物有限公司，上海 200237

收稿日期:2008-05-30 出版日期:2008-05-31 发布日期:2008-05-30
基金资助:
上海市科学基金资助项目（054909002）

Study on Sequence Variation of Mitochondrial D-loop Gene and Polymorphism among four populations of Reed Vole (Microtus fords)

1. Shanghai Quality Monitoring C enter for Laboratory Animal, Shanghai Laboratory Animal Research Center, Shanghai 201203, China; 2. Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China;3. Shanghai Xipuer-Bikai Laboratory Animal Limited Company, shanghai 201203, China

Received:2008-05-30 Online:2008-05-31 Published:2008-05-30

摘要/Abstract

摘要： 目的探讨我国不同地区东方田鼠线粒体DNA D-loop多态性。方法对四个种群东方田鼠线粒体DNA（mt DNA）D-loop片段进行测序，并对测序结果用Clustal W软件进行比对，通过MEGA Version 3.1软件构建基于平均遗传距离的NJ（neighbor-joining，邻接法）分子系统进化树，用Dnasp 4.0分析所有序列的多态位点、核苷酸多样性、单倍型数目、单倍型多样性以及进行Tajima’s中性显著性检验。结果四个种群34个东方田鼠个体的mt DNA D-loop区全序列共发现19个单倍型，单倍型比例为55.88%，单倍型多样性和核苷酸多样性分别为0.934±0.024和0.04334±0.00285。以台湾田鼠（Microtus kikuchii）线粒体全序列（GenBank登录号AF348082）为对照，共检测到核苷酸变异位点172个，约占核苷酸总数的18.66%。由分子系统进化树可发现所有34只东方田鼠分为两大类，小体型黑龙江东方田鼠为一类，其他三个种群东方田鼠为另外一类，在这一类别中，又有两个分支，其中所有宁夏种群鼠及3只大体型黑龙江种群鼠为一个分支，所有洞庭湖种群鼠和其他5只大体型黑龙江种群鼠为另一个分支。结论我国东方田鼠在mt DNA D-loop片段存在种内遗传多态性，种群内和种群间都存在一定的遗传变异，并且黑龙江小体型种群东方田鼠表现出与其他三种群东方田鼠更大的差异水平。

关键词: 东方田鼠, 线粒体DNA（mt, DNA）D-loop区, 遗传多态性

Abstract: Objectives To discuss the genetic polymorphisms of the mitochondrial DNA (mtDNA) D-loop among four populations of Reed voles (Microtus fortis) in different regions of China. Methods A total of 34 specimens representing 4 populations were sequenced for the entire D-loop gene (922bp). All sequences were aligned by Clustal W software, and The neighbor-joining (NJ) phylogenetic tree was formed by the MEGA Version 3.1 software. The polymorphic site, haplotype diversity and nucleotide diversity were analyzed by Dnasp 4.0 software. Result 19 haplotypes were determined among 34 individuals, the haplotype ratio was 55.88%. The haplotype diversity and nucleotide diversity was 0.934士0.024 and 0.04334士0.00285 respectively. In reference to mtDNA D-loop sequences of Microtus kikuchii (GenBank AF348082) as a control, there were 172 genetic mutation sites, accounting for 18.66% of 922 bp sequence.The phylogenetic trees showed that 34 vole individuals clustered into two distinct branches: voles from Heilongjiang (smaller) were one branch, the other three vole populations comprised as the other branch, and in this branch, there were two sub-branches, all vole from Ningxia and some voles from Heilongjiang (lager) buildup one sub-branch, voles from Hunan and the rest voles from Heilongjiang (lager) belonged to the other sub-branch. Conclusion These findings suggested that the mtDNA D-loop in Reed voles from China existed informative variations within the four populations, there were some genetic diverse among intra- and inter-populations of Reed voles, but voles from Heilongjiang (smaller) showed more diversity from the other three populations.

Key words: Reed vole (Microtus fortis)； mitochondrial DNA (mtDNA), D-loop, genetic polymorphism

谢建云1，2，冯洁1，柏熊3，胡建华1，高诚1. 四种群东方田鼠线粒体DNA[J]. , 2008, 28(5): 299-303.

Xie Jian-yun1’2，Feng Jie1，Bai Xiong3, Hu Jian-hua1，Gao Cheng1 . Study on Sequence Variation of Mitochondrial D-loop Gene and Polymorphism among four populations of Reed Vole (Microtus fords)[J]. , 2008, 28(5): 299-303.

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四种群东方田鼠线粒体DNA

Study on Sequence Variation of Mitochondrial D-loop Gene and Polymorphism among four populations of Reed Vole (Microtus fords)

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