DDTs对唐鱼仔鱼的急性毒性及安全浓度评价

›› 2007, Vol. 27 ›› Issue (2): 123-126.

DDTs对唐鱼仔鱼的急性毒性及安全浓度评价

华南师范大学生命科学学院，广州 510631

收稿日期:2006-10-08 出版日期:2007-02-28 发布日期:2007-02-28
基金资助:
农业部渔业生态环境重点开放实验室开放基金和广东省渔业生态环境重点开放实验室开放基金资助（2005-09）；广东省科技计划项目资助（2006B36601008）

Acute Toxicity and Safety Assessment of DDTs to White Cloud Mountain Minnow Tanichthys albonubes larval stages

1. Department of Laboratory Animal science, FuDan University, Shanghai 200032, China;2 Shanghai Laboratory Animal Center, The Chinese Academy of Sciences, Shanghai 201615, China;3. Department of Surgery, Hua Dong Hospital, Shanghai 200040, China

Received:2006-10-08 Online:2007-02-28 Published:2007-02-28

摘要/Abstract

摘要： 目的研究有机氟农药滴滴涕（dichlorodiphenyltrichloroethanes，DDTs）对唐鱼的急性毒性，评价水环境中DDTs对鱼类的影响，为渔业部门制定水质标准提供参考.方法使用浸浴法以0.0125、0.025、0.045、0.08和0.14mg/L五个DDTs浓度为唐鱼染毒，计算其对仔鱼的急性毒性及安全浓度.结果 DDTs对唐鱼仔鱼为剧毒物质，24、48 h的LC₅₀分别为0.243、0.049 mg/L，其安全质量浓度为0.040 mg/L.结论唐鱼对DDTs敏感，可以作为一种较理想的环境污染指示生物.

关键词: 滴滴涕, 唐鱼, 急性毒性, 安全浓度

Abstract: Objective To study the therapeutic effect of combining angiostatin gene therapy with peritoneal chemotherapy with 5-FU on cachexia in nude mice bearing with colorectal carcinoma metastasis in abdominal cavity. Methods Angiostatin gene was cloned into adenovirus with method of RT-PCR. LoVo cell suspension was i.p to 40 nude mice. The mice were treated with combining angiostatin gene therapy and 5-FU peritoneal chemotherapy or others. Observation were made on the improvement of physiological conditions of the cachetic nude mice after treatment, and the serum levels of TNF-a and IL-1 were assayed by ELISA method. Result Compared with the controlled groups, the group of combining therapy was less loss of body mass, higher food intake, better mental condition and lower levels of TNF-a and IL-1. Conclusion Combining angiostatin gene therapy and 5-FU peritoneal chemotherapy has beneficial effect on cachexia in nude mice bearing with colorectal carcinoma in abdominal cavity.

Key words: Angiostatin, Colorectal Carcinoma, 5-FU, Gene Therapy, Cachexia

杨志聪，姚静，方展强. DDTs对唐鱼仔鱼的急性毒性及安全浓度评价[J]. , 2007, 27(2): 123-126.

YANG Zhi-cong,YAO Jing,FANG Zhan-qiang. Acute Toxicity and Safety Assessment of DDTs to White Cloud Mountain Minnow Tanichthys albonubes larval stages[J]. , 2007, 27(2): 123-126.

[1]	赖素群, 余露军, 吴美慧, 曾进, 李建军. 紫海胆急性毒性试验方法标准化探讨[J]. 实验动物与比较医学, 2020, 40(1): 65-69.
[2]	李建军, 黄韧. 不同发育阶段诸氏鲻虾虎鱼对水污染物的敏感性比较[J]. 实验动物与比较医学, 2013, 33(5): 354-357.
[3]	张燕, 范文玺, 徐艺玫, 史深, 贵友军, 燕顺生. β-咔啉类衍生物对BALB/c裸小鼠肝癌皮下移植瘤生长的影响[J]. 实验动物与比较医学, 2013, 33(4): 290-295.
[4]	程树军, 史光华. 化学品鱼类急性毒性的决策树及替代试验[J]. 实验动物与比较医学, 2012, 32(2): 152-155.
[5]	孙鹏, 李鹏, 张旭瑞, 乔伟伟, 赵霞, 张云鹏. 量子点的急性毒性及动力学初步观察[J]. 实验动物与比较医学, 2011, 31(4): 255-258.
[6]	王颖, 孙丽萍, 魏社林, 陈琳, 黄韧. 四种水生动物对GM-2消油剂的急性毒性反应[J]. 实验动物与比较医学, 2011, 31(4): 259-263.
[7]	张薇1，张钟广2，许崇辉3，欧阳少伦3，赵勇1，胡海勋1，黎福荣1，林以理1，钟女奇1，庄少慧1，邹移海2. 三聚氰胺急性毒性及中药三金汤的清除作用[J]. , 2010, 30(3): 188-192.
[8]	邝少松，赵士海，谢超敏，刘盛来，张志. 垫料水提取物的急性毒性及微核试验[J]. , 2008, 28(4): 269-270.
[9]	刘志勇, 李启富, 周银平, 戴黎光, 周明, 刘琳, 田泽敏, 包锦英, 喻荣珍, 杨新跃. 苏云金杆菌的急性毒性及致敏实验观察[J]. , 2004, 24(3): 157-159.