关键词: 川崎病(KD), 冠状动脉炎, 内皮细胞, 线粒体, 小鼠

Abstract: Objective To observe the dynamic changes of morphology of vascular endothelial cell mitochondria in mice with immunologic coronaritis,in order to simulate the corresponding changes in endothelial cells and mitochondrial with coronaritis of Kawasaki disease.Methods C57BL/6 mice were randomly divided into experimental group (n=30) and control group (n=30).Mice were peritonealy injected with Candida albicans water soluble fraction or phosphate buffer (PBS) for 5 d.After the last injection of 24 h,3 d,7 d,14 d and 28 d,6 mice in each group were sacrificed to detect the blood routine and the level of interleukin (IL)-6 and tumor necrosis factor (TNF)-αin the plasma.Cardiac and coronary artery specimens were examined by hematoxylin eosin (HE) staining.The structure of mitochondria in the endothelial cells of coronary artery was observed by electron microscopy.Results The levels of blood white blood cells,platelets,plasma IL-6 and TNF-αin the experimental group were increased at the time of 3 d and 7 d after injection (P<0.05).The histopathology of the early stage in coronary artery inflammation (3 d),the endothelial cells were swollen.The fracture of elastic fiber and hemangioma appeared after the mid-term (14 d).Under the perspective of electron microscopy,coronary artery endothelial cells showed swelling and vacuolar degeneration at middle stage (24 h,3 d,7 d and 14 d),and the number of mitochondrion decreased,the crest blurred,and the electron density decreased.Conclusion The mouse model constructed can simulate characteristics similar to Kawasaki disease immunity coronaritis,suggesting that mitochondria in the endothelial cells involved in the immune process of coronary arteritis.

Key words: Kawasaki disease, Coronaritis, Endothelial cells, Mitochondria, Mice