硒对氟致大鼠肾小管上皮细胞线粒体膜电位改变的拮抗作用

doi:10.3969/j.issn.1674-5817.2017.03.002

摘要/Abstract

摘要： 目的探究硒对氟致大鼠肾小管上皮细胞(NRK-52E细胞)线粒体膜电位改变的拮抗作用。方法实验共设4组: 染氟组(5 mg/L和20 mg/L的氟化钠)、染硒组(0.0171 mg/L和0.0342 mg/L的亚硒酸钠)、硒干预组(氟化钠和亚硒酸钠联合干预)和对照组,按照分组干预NRK-52E细胞48 h。流式细胞仪检测其线粒体膜电位,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)和总抗氧化力(T-AOC)测试盒测定酶的活性。结果与对照组比,随着氟化钠浓度的升高,染氟组NRK-52E细胞线粒体膜电位下降(P<0.05或P<0.01)且SOD、T-AOC、GSH-PX和CAT酶活性均降低(P<0.05或P<0.01)。与染氟组相比,相对应的硒干预组线粒体膜电位升高(P<0.01)。与20 mg/L的染氟组比较,相对应硒干预组的SOD、T-AOC、GSH-PX和CAT酶活性均升高(P<0.05)。与0.0171 mg/L染硒组相比,相对应的硒干预组线粒体膜电位降低(P<0.01)且T-AOC、GSH-PX和CAT酶活性均降低(P<0.01或P<0.05)。5 mg/L加氟组与加硒组相比,线粒体膜电位显著变化(P<0.01), 但SOD、GSH-PX、CAT、T-AOC酶活性均无显著变化。20 mg/L加氟组与加硒组相比,线粒体膜电位具有显著性差异(P<0.01)且SOD、GSH-PX、CAT、T-AOC酶活性均有显著性差异(P<0.01)。结论一定浓度的硒可以拮抗氟诱导大鼠肾小管上皮细胞线粒体膜电位下降,可能通过恢复抗氧化酶活性实现。

关键词: 氟, 硒, 肾小管上皮细胞, 线粒体膜电位, 抗氧化酶

Abstract: Objective To study effects of fluoride on the change of mitochondrial membrane potential in rat renal tubular epithelial cells (NRK-52E cells). Methods The experiments were divided into 4 groups: the control group, the fluoride group, the selenium group and the selenium intervention group.NRK-52E cells were cultured with fluoride and selenium respectively in various concentration, and fluoride and selenium combination for 48 h. (5 mg/L or 20 mg/L NaF、0.0171 mg/L or 0.0342 mg/L Na₂SeO₃). The mitochondrial membrane potential were detected by flow cytometry. The activity of enzymes and the content of free radicals were examined by relevant reagent kit, such as superoxide dismutase(SOD) reagent kit. Results Compared with the control group, the mitochondrial membrane potential decreased (P<0.05 or P<0.01) and SOD, total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX) and catalase (CAT) enzyme activities decreased (P<0.05) with the increase of the concentration of sodium fluoride.The mitochondrial membrane potential of the selenium intervention group was increased (P<0.01) compared with that of the fluoride exposed group.Compared with the fluoride group of 20 mg/L, the relative selenium intervention group SOD, T-AOC, GSH-PX and CAT activities were increased (P<0.01). Compared with the selenium group of 0.0171 mg/L, the relative selenium intervention group the mitochondrial membrane potential decreased (P<0.01) and SOD, T-AOC, GSH-PX and CAT enzyme activities decreased (P<0.05 or P<0.01). 5 mg/L the fluoride group compared with the selenium group, the mitochondrial membrane potential changed significantly (P<0.01). But for SOD, T-AOC, GSH-PX and CAT activities ,there were no significant changed. 20 mg/L the fluoride group compared with the selenium group, the mitochondrial membrane potential changed significantly (P<0.01). SOD, T-AOC, GSH-PX and CAT activities also changed significantly (P<0.01). Conclusion The certain concentration of selenium could induce the decrease of mitochondrial membrane potential in NRK-52E cells induced by fluoride, that may be achieved by restoring the activities of activities.

Key words: Sodium fluoride, Selenium, Rat renal tubular epithelial cells, Mitochondrial membrane

中图分类号:

Q95-33

常凯, 王裕, 庞文彪, 高继萍, 陈朝阳, 宋国华. 硒对氟致大鼠肾小管上皮细胞线粒体膜电位改变的拮抗作用[J]. 实验动物与比较医学, 2017, 37(3): 179-184.

CHANG Kai, WANG Yu, PANG Wen-biao, GAO Ji-ping, CHEN Zhao-yang, SONG Guo-hua. Antagonistic Effect of Selenium on Change of Mitochondrial Membrane Potential of NRK-52E Cells Induced by Sodium Fluoride[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 179-184.

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