关键词: 氟化钠, 软骨细胞, 糖胺多糖, II型胶原

Abstract: Objective To observe the effect of secretion of GAG and II type collagen of excess fluoride on in vitro chondrocytes of rats. Methods Chondrocytes of rats was separated from the 2-month old female rats, and cultured in the cell bottles. After identification, six groups were designed in the research including control group and NaF group (6.25 µmol/ml, 12.50 µmol/ml, 25.00 µmol/ml, 50.00 µmol/ml and 100.00 µmol/ml). After 6 days of administration, GAG contents were measured. Alcian Blue dye and II type collagen immunohistochemistry dye were also performed on the in vitro chondrocytes of rats. Results After 6 days of treatment, there were no significant difference of GAG contents of cell culture medium between NaF groups and control group (P>0.05). Alcian Blue dye showed that secretion of GAG content of all groups did not change after administered by NaF. But II type collagen immunohistochemistry dye showed that scores of II type collagen immunohistochemistry dye increased according to dosage of NaF leveled up (P<0.05). Conclusions NaF may not affect the content of GAG secreted by the chondrocytes of rats in vitro, but can affect the secretion of II type collagen.

Key words: NaF, Chondrocytes, GAG, II type collagen