三种饲料建立大鼠缺铁性贫血模型的比较

doi:10.3969/j.issn.1674-5817.2016.06.006

实验动物与比较医学 ›› 2016, Vol. 36 ›› Issue (6): 433-436.DOI: 10.3969/j.issn.1674-5817.2016.06.006

三种饲料建立大鼠缺铁性贫血模型的比较

丁广宇, 潘绵立, 胡寒, 刘艺, 沈龙海

中国医药工业研究总院上海医药工业研究院, 上海市生物物质成药性评价专业技术服务平台, 上海 200437

收稿日期:2016-08-02 出版日期:2016-12-25 发布日期:2016-12-25
作者简介:丁广宇(1992-), 男, 硕士研究生, 研究方向: 方向为免疫药理学。E-mail: 1293032013@qq.com

Comparative Study on Iron Deficiency Anemia Rats Model by Three Different Formula Feeds

DING Guang-yu, PAN Mian-li, HU Han, LIU Yi, SHEN Long-hai

China State Institute of Pharmaceutical Industry, Shanghai Professional and Technical Service Center for Biological Material Druggability Evaluation, Shanghai 200437, China

Received:2016-08-02 Online:2016-12-25 Published:2016-12-25

摘要/Abstract

摘要： 目的探讨三种不同饲料配方对建立Wistar大鼠缺铁性贫血模型的影响。方法将3~4周龄Wistar大鼠分为4组,即A、B、C和正常对照组, A、B、C组分别饲喂3种不同配方饲料, 正常对照组饲喂正常饲料。实验持续35 d, 实验期间每周称量各组大鼠体质量及摄食量同时观察大鼠行为、外观体征等变化。35 d后取血, 检测相关血常规指标包括血红蛋白(Hb)含量、红细胞压积等。结果 A组大鼠Hb含量降到90 g/L以下, 且行为和体态表现也符合缺铁贫血特征, 达到造模要求; B组大鼠Hb含量没有降低; C组大鼠Hb含量有所降低, 但未达到90 g/L以下。结论 A组饲料配方可以成功建立大鼠缺铁性贫血模型,B、C组配方均不适用于缺铁性模型的建立。

关键词: 缺铁性贫血, Wistar大鼠, 血红蛋白(Hb)

Abstract: Objective To explore the effect on iron deficiency anemia rat model induced by 3 different formula feeds. Method Wistar rats at age of 3~4 weeks were randomly divided into 4 groups: group A, group B, group C and control group. The groups A, B, C were fed with 3 different formula feeds respectively, and control group was fed with normal feed. Experiment lasts for 35 days, the body weight and food intake of rats in each group were measured every week. Meanwhile, behavior and appearance sign was noted during the experiment. Rat抯 blood was collected and tested after 35 days, including hemoglobin, hematocrit, etc. Results Hemoglobin(Hb) concentration of group A decreased to 90 g/L and morphological features met the requirments. Hb in group B did not decrease; Hb in group C decreased a little but no less than 90 g/L. Conclusion The formular A feed can establish iron deficiency anemia model successfully, formula B and C are not applicable for the model building.

Key words: Iron deficiency anemia, Wistar rats, Hemoglobin(Hb)

中图分类号:

Q95-33

丁广宇, 潘绵立, 胡寒, 刘艺, 沈龙海. 三种饲料建立大鼠缺铁性贫血模型的比较[J]. 实验动物与比较医学, 2016, 36(6): 433-436.

DING Guang-yu, PAN Mian-li, HU Han, LIU Yi, SHEN Long-hai. Comparative Study on Iron Deficiency Anemia Rats Model by Three Different Formula Feeds[J]. Laboratory Animal and Comparative Medicine, 2016, 36(6): 433-436.

[1]	侯敏博, 崔甜甜, 苏娜瑛, 张苗苗, 焦永敏, 严建燕, 汪溪洁, 大平东子. 一例Han-Wistar大鼠垂体细胞瘤的病理学分析[J]. 实验动物与比较医学, 2023, 43(6): 654-658.
[2]	许冰馨, 范凯健, 王婷玉, 陈慧瑾. 地塞米松对胶原诱导性关节炎大鼠模型软骨退化的改善作用[J]. 实验动物与比较医学, 2022, 42(5): 416-422.
[3]	魏杰, 左琴, 王洪, 李欢, 周佳琪, 光姣娜, 范涛, 刘佐民, 付瑞, 岳秉飞. 基于团体标准T/CALAS 21—2017的Wistar大鼠微卫星DNA群体遗传质量分析[J]. 实验动物与比较医学, 2021, 41(6): 528-534.
[4]	吕晓君, 吴森, 张菊, 徐小玲, 潘望平, 李厚钢, 王平慧, 何开勇. 用高热量饲料建立大鼠肥胖模型的实验研究[J]. 实验动物与比较医学, 2020, 40(5): 374-.
[5]	肖成荣, 谭洪玲, 马增春, 王宇光, 王德文, 孙敬翔, 高月. 老年Wistar大鼠自发性肿瘤及血液生化检测[J]. 实验动物与比较医学, 2014, 34(4): 324-329.
[6]	徐艺玫1，刘晓峰2，燕顺生1，熊进2，侯岩岩1，陈欣如1，麦丽开1，杜勇2. 红花籽油软胶囊短期饲喂大鼠的安全性评价[J]. , 2007, 27(3): 198-200.
[7]	黄小琼, 邝少松, 王刚, 饶子亮, 陆春丽, 刘盛来. 不同性别、年龄Wistar大鼠常规血液生化值测定[J]. , 2006, 26(4): 245-246.
[8]	王立, 胡岩松, 于学慧, 邢志淑, 尹志圣. 大鼠对蛋白质的需求初探[J]. , 1998, 18(3,4): 170-172.
[9]	李善如, 王冬平, 耿志贤, 江其辉. 老龄Wistar大鼠脏器重量及血液生化值测定[J]. , 1995, 15(3): 179-181.

三种饲料建立大鼠缺铁性贫血模型的比较

Comparative Study on Iron Deficiency Anemia Rats Model by Three Different Formula Feeds

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 9

编辑推荐

Metrics

本文评价