SCID Beige与NOD.CB17-Prkdcscid小鼠主要免疫指标测定

doi:10.3969/j.issn.1674-5817.2016.05.008

实验动物与比较医学 ›› 2016, Vol. 36 ›› Issue (5): 365-368.DOI: 10.3969/j.issn.1674-5817.2016.05.008

所属专题：实验动物资源开发与利用

SCID Beige与NOD.CB17-Prkdcscid小鼠主要免疫指标测定

袁水娟¹, 施国君¹, 唐慧青¹, 许文静¹, 符杰², 施美莲²

1.上海灵畅生物科技有限公司, 上海 201402;
2.上海斯莱克实验动物有限责任公司, 上海 201615

收稿日期:2016-06-17 出版日期:2016-10-25 发布日期:2016-10-25
作者简介:袁水娟(1981-), 女, 畜牧师, 从事实验动物生产及管理。E-mail: ysjdyx123@163.com
基金资助:
上海市科委实验动物研究专项(13140900500)

Detection of Main Immune Indexes in SCID. Beige and NOD.CB17-Prkdcscid Mice

YUAN Shui-juan¹, SHI Guo-jun¹, TANG Hui-qing¹, XU Wen-jing¹, FU Jie², SHI Mei-lian²

1. Shanghai Lingchang Bio-tech Company Limited, Shanghai 201402, China;
2. Shanghai SLAC Laboratory Animal Company Limited, Shanghai 201615, China

Received:2016-06-17 Online:2016-10-25 Published:2016-10-25

摘要/Abstract

摘要： 目的测定SCID Beige (简称SCID.BG)小鼠与NOD.CB17-Prkdcscid (简称NOD.SCID)小鼠主要免疫球蛋白(Ig)、补体(C)、免疫细胞指标,分析两种免疫缺陷小鼠在免疫方面差异。方法选择4周龄、8周龄、16周龄的SCID.BG小鼠、NOD.SCID小鼠各30只(雌雄各半),测定主要免疫球蛋白、补体指标; 选择8周龄的SCID.BG小鼠、NOD.SCID小鼠各14只(雌雄各半),测定T细胞及亚群、B细胞、NK细胞免疫指标。结果 3个年龄段的SCID.BG在IgG水平上显著低于NOD.SCID,在IgA水平上显著高于NOD.SCID; 4周龄、16周龄的SCID.BG在C4水平上显著高于NOD.SCID; 8周龄 SCID.BG小鼠与NOD.SCID小鼠在IgG水平上均显著低于其他两个周龄; T细胞亚群中,8周龄 SCID.BG的CD4⁺CD8⁺百分比显著低于NOD.SCID; SCID.BG小鼠与NOD.SCID小鼠同品系同周龄在性别上各项指标均无显著差异。结论 SCID.BG与NOD.SCID抗体、补体、免疫细胞水平含量均远低于正常小鼠的水平,同属于T、B、NK重度免疫缺陷动物,两品系同周龄同性别比较各项免疫指标各有高低,在实验中可以根据不同的研究目的选择动物。

关键词: SCID Beige (SCID.BG)小鼠, NOD.CB17-Prkdcscid (NOD.SCID)小鼠, 免疫球蛋白(Ig), 补体 (C), 免疫细胞

Abstract: Objective To measure the immunoglobulin, complement and main immune indexes in SCID. Beige and NOD.SCID mice and analyze their difference. Methods Thirty SCID.Beige and thiry NOD.SCID mice, half male and female, at the age of 4 weeks, 8 weeks and 16 weeks were selected for detecting the main immunoglobulin and complement indexes. Another fourteen SCID.Beige and fourteen NOD.SCID mice, half male and female, at the age of 8 weeks were also selected for detecting the T, B lymphocyte function and NK cell activity. Results The IgG of SCID.Beige at all the age stages were significantly lower than those of NOD.SCID, but the IgA were significantly higher than those of NOD. SCID.The serum C4 of SCID.Beige mice at the age of 4 weeks and 16 weeks were significantly higher than those of NOD.SCID. The IgG of SCID.Beige and NOD.SCID mice at the age of 8 weeks were significantly lower than the other two age stages. In the T cell subsets, the CD4⁺CD8⁺ percentages of SCID.Beige mice at the age of 8 weeks were significantly lower than those of NOD.SCID. Among the same sex and age group of NOD.SCID and SCID.BG, the immune indexes show no obvious differences. Conclusions The antibodies, complement and immune cell levels of SCID.Beige and NOD.SCID are significantly lower than normal mice. They all belong to severe immunodeficiency animals. Among the same age and sex group of the two strains, the immune indexes were different. So the animals should be selected according to the research purpose in the experiments.

Key words: SCID.Beige mice, NOD.CB17-Prkdcscid mice, Immunoglobulin, Complement, Immune cells

中图分类号:

Q95-33

袁水娟, 施国君, 唐慧青, 许文静, 符杰, 施美莲. SCID Beige与NOD.CB17-Prkdcscid小鼠主要免疫指标测定[J]. 实验动物与比较医学, 2016, 36(5): 365-368.

YUAN Shui-juan, SHI Guo-jun, TANG Hui-qing, XU Wen-jing, FU Jie, SHI Mei-lian. Detection of Main Immune Indexes in SCID. Beige and NOD.CB17-Prkdcscid Mice[J]. Laboratory Animal and Comparative Medicine, 2016, 36(5): 365-368.

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SCID Beige与NOD.CB17-Prkdcscid小鼠主要免疫指标测定

Detection of Main Immune Indexes in SCID. Beige and NOD.CB17-Prkdcscid Mice

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