裸鼹鼠骨骼标本的制作及其形态学观察

doi:10.3969/j.issn.1674-5817.2013.06.017

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (6): 493-498.DOI: 10.3969/j.issn.1674-5817.2013.06.017

• 裸鼹鼠硏究专题 • 上一篇

裸鼹鼠骨骼标本的制作及其形态学观察

赵善民, 肖邦, 余琛琳, 林丽芳, 蔡丽萍, 张璐, 袁子彦, 孙智远, 孙伟, 汤球, 崔淑芳

第二军医大学实验动物中心, 上海200433

收稿日期:2013-07-15 出版日期:2013-12-25 发布日期:2013-12-25
作者简介:赵善民(1986-), 男, 硕士, 助教
基金资助:
上海市科技发展基金项目(No. 11140901700)、 (No.12140900200)与(No.13140900400)联合资助

Making of Skeleton Specimens and Morphological Observation in Naked Mole Rat

ZHAO Shan-min, XIAO Bang, YU Chen-Lin, LIN Li-fang, CAI Li-ping, ZHANG Lu, YUAN Zi-yan, SUN Zhi-yuan, SUN Wei, TANG Qiu, CUI Shu-fang

Laboratory Animal Centre , Second Military Medical University, Shanghai 200433, China

Received:2013-07-15 Online:2013-12-25 Published:2013-12-25

摘要/Abstract

摘要： 目的采用传统方法制备裸鼹鼠骨骼标本,对其骨骼系统进行形态学观察。方法利用水煮法制作裸鼹鼠骨架,与C57BL/6J小鼠骨骼标本比较研究裸鼹鼠骨骼特点,并观察裸鼹鼠股骨三维结构。结果所制作的裸鼹鼠骨架完整洁净, 没有骨质损伤,裸鼹鼠头骨、椎骨、肋骨、四肢骨均具有适应于穴居习性的特征。结论裸鼹鼠的骨骼具有典型的适应掘穴生活的特征。

关键词: 裸鼹鼠, 骨骼, 制作, 形态学观察

Abstract: Objective The traditional technique for making skeleton specimen was applied to study the morphology of the skeleton of the naked mole rat. Methods Skeleton specimens of the naked mole rat were made and compared with inbred mice strains C57 BL/6J. Then the three-dimensional structure of the femur was observed in the naked mole rat. Results Skeletons of naked mole rats were made without damaged. The traits of skull, spine, ribs, sternum and limbs of the naked mole rat, which are adapt to their fossorial habits, are discovered. Conclusion Skeletons of the naked mole rat have typical characteristics of adaptation to their fossorial habits.

Key words: Heterocephalus glaber, Skeleton specimens, Making, Morphological Observation

赵善民, 肖邦, 余琛琳, 林丽芳, 蔡丽萍, 张璐, 袁子彦, 孙智远, 孙伟, 汤球, 崔淑芳. 裸鼹鼠骨骼标本的制作及其形态学观察[J]. 实验动物与比较医学, 2013, 33(6): 493-498.

ZHAO Shan-min, XIAO Bang, YU Chen-Lin, LIN Li-fang, CAI Li-ping, ZHANG Lu, YUAN Zi-yan, SUN Zhi-yuan, SUN Wei, TANG Qiu, CUI Shu-fang. Making of Skeleton Specimens and Morphological Observation in Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2013, 33(6): 493-498.

参考文献

[1] Johansen K, Lykkeboe G, Weber RE, et al.Blood respiratory properties in the naked mole rat Heterocephalus glaber, a mammal of low body temperature[J]. Respir Physiol, 1976,28(3):303-314.
[2] O’Connor TP, Lee A, Jarvis JU, et al. Prolonged longevity in naked mole-rats: age-related changes in metabolism, body composition and gastrointestinal function[J]. Comp Biochem Physiol A Mol Integr Physiol, 2002, 133(3):835-842.
[3] Peterson BL, Larson J, Buffenstein R, et al.Blunted neuronal calcium response to hypoxia in naked mole-rat hippocampus[J]. PLoS One, 2012, 7(2):e31568.
[4] Peterson BL, Park TJ, Larson J.Adult naked mole-rat brain retains the NMDA receptor subunit GluN2D associated with hypoxia tolerance in neonatal mammals[J]. Neurosci Lett,2012, 506(2):342-345.
[5] 林丽芳, 赵懿宁, 赵善民, 等.裸鼹鼠与C57BL6小鼠自噬调节的比较研究[J]. 实验动物与比较医学, 2013, 33(4):301-305.
[6] 孙伟, 汤球, 赵善民, 等. 裸鼹鼠人工饲养繁殖初步研究[J].实验动物与比较医学, 2013, 33(4):296-300.
[7] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肺脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):378-382.
[8] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肝脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):373-377.
[9] 赵善民, 赵懿宁, 汤球, 等. 裸鼹鼠胸腺、脾脏及淋巴结解剖学、组织学与超微结构研究[J]. 实验动物与比较医学,2013, 33(5):395-399.
[10] 赵善民, 崔淑芳. 裸鼹鼠生物学特性的研究进展[J]. 实验动物与比较医学, 2013, 33(5):400-405.
[11] Henry EC, Dengler-Crish CM, Catania KC.Growing out of a caste-reproduction and the making of the queen mole-rat[J].J Exp Biol, 2007, 210(Pt 2):261-268.
[12] Stanley LA, Devereux TR, Foley J, et al.Proto-oncogene activation in liver tumors of hepatocarcinogenesis-resistant strains of mice[J]. Carcinogenesis, 1992, 13(12):2427-2433.
[13] Kushida M, Kamendulis LM, Peat TJ, et al.Dose-related induction of hepatic preneoplastic lesions by diethylnitrosa-
mine in C57BL/6 mice[J]. Toxicol Pathol, 2011, 39(5):776-786.
[14] 蒙绍权, 李桂芬, 黄鸿宣, 等. 版纳鱼螈的骨骼系统[J]. 动物学杂志, 2006, 41(4):100-106.
[15] Legrand E, Audran M, Guggenbuhl P, et al.Trabecular bone microarchitecture is related to the number of risk factors and etiology in osteoporotic men[J].Microsc Res Tech, 2007, 70(11):952-959.
[16] Pinto M, Jepsen KJ, Terranova CJ, et al.Lack of sexual dimorphism in femora of the eusocial and hypogonadic naked mole-rat: a novel animal model for the study of delayed puberty on the skeletal system[J]. Bone, 2010, 46(1):112-120.
[17] Ward WE, Kim S, Chan D, et al.Serum equol, bone mineral density and biomechanical bone strength differ among four mouse strains[J]. J Nutr Biochem, 2005, 16(12):743-749.
[18] Zhang P, Jiang C, Yokota H.Lengthening of mouse hind limbs with local administration of insulin-like growth factor 2[J]. J Chin Med Assoc, 2012, 75(10):494-500.
[19] Monir AU, Gundberg CM, Yagerman SE, et al.The effect of lead on bone mineral properties from female adult C57/BL6 mice[J].Bone, 2010, 47(5):888-894.
[20] Buffenstein R, Jarvis JU, Opperman LA, et al.Subterranean mole-rats naturally have an impoverished calciol status, yet synthesize calciol metabolites and calbindins[J]. Eur J Endocrinol, 1994, 130(4):402-409.
[21] Buffenstein R, Sergeev IN, Pettifor JM.Vitamin D hydroxylases and their regulation in a naturally vitamin D-deficient subterranean mammal, the naked mole rat (Heterocephalus glaber)[J].J Endocrinol, 1993, 138(1):59-64.
[22] Pitcher T, Pettifor JM, Buffenstein R.The effect of dietary calcium content and oral vitamin D3 supplementation on mineral homeostasis in a subterranean mole-rat Cryptomys damarensis[J]. Bone Miner, 1994, 27(2):145-157.
[23] Pitcher T, Sergeev IN, Buffenstein R.Vitamin D metabolism in the Damara mole-rat is altered by exposure to sunlight yet mineral metabolism is unaffected[J]. J Endocrinol, 1994, 143(2):367-374.

裸鼹鼠骨骼标本的制作及其形态学观察

Making of Skeleton Specimens and Morphological Observation in Naked Mole Rat

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	冯延, 武文卿, 张静远, 李煜, 李壘辰, 袁征, 崔淑芳. 裸鼹鼠皮肤成纤维细胞外泌体的分离及鉴定[J]. 实验动物与比较医学, 2020, 40(6): 506-512.
[2]	张静远, 张倩倩, 赵懿宁, 杨蓉, 张成财, 贺辰囧, 袁征, 崔淑芳. ⁶⁰Co γ射线辐射对裸鼹鼠肾脏、肺脏以及骨骼肌的影响[J]. 实验动物与比较医学, 2020, 40(6): 513-518.
[3]	杨蓉, 赵懿宁, 杨文静, 赵善民, 柴宇静, 张成财, 袁征, 崔淑芳. ⁶⁰Co γ射线辐射对裸鼹鼠脾脏的影响[J]. 实验动物与比较医学, 2020, 40(6): 519-522.
[4]	张成财, 刘尹航, 陈超, 江文正. 脂多糖对裸鼹鼠肺脏组织影响的初探[J]. 实验动物与比较医学, 2020, 40(6): 523-527.
[5]	李煜, 刘尹航, 陈超. 裸鼹鼠心脏对60Coγ射线辐射耐受的初步研究[J]. 实验动物与比较医学, 2020, 40(5): 415-.
[6]	许振坤, 任丽玮, 原涛. 高脂饲喂结合颈总动脉球囊损伤制作胰岛素抵抗兔动脉粥样硬化模型[J]. 实验动物与比较医学, 2020, 40(1): 28-32.
[7]	杨文静, 李煜, 冯延, 李壘辰, 许月榕, 刘攀, 林丽芳, 孙伟, 崔淑芳. 裸鼹鼠海马神经元纯化模型建立及其低氧耐受特性机制的初步研究[J]. 实验动物与比较医学, 2019, 39(6): 467-472.
[8]	林丽芳, 张成财, 李煜, 张倩倩, 陈超, 李壘辰, 杨文静, 李莉, 崔淑芳. Poly I:C刺激对裸鼹鼠和小鼠巨噬细胞PKR/eIF2α信号通路影响的比较研究[J]. 实验动物与比较医学, 2019, 39(6): 473-478.
[9]	陈超, 丛薇, 杨文静, 崔淑芳. 裸鼹鼠与小鼠抗辐射能力比较研究[J]. 实验动物与比较医学, 2019, 39(6): 479-483.
[10]	陈超, 丛薇, 杨文静, 崔淑芳. 裸鼹鼠抗化学诱癌因素特性的研究[J]. 实验动物与比较医学, 2019, 39(6): 484-488.
[11]	张雅荃, 杨文静, 林丽芳, 阮文婕. 睡眠剥夺对小鼠生长发育影响初探[J]. 实验动物与比较医学, 2018, 38(3): 212-216.
[12]	程继帅, 袁征, 杨文静, 李壘辰, 丛薇, 孙伟, 柏晓松, 崔淑芳. 裸鼹鼠血清中免疫球蛋白的分离纯化及含量的检测[J]. 实验动物与比较医学, 2017, 37(5): 414-417.
[13]	刘乾, 刘松, 徐卫国, 管思彬, 郭雪君. 泛素特异性蛋白酶19对烟熏诱导慢性阻塞性肺疾病大鼠模型骨骼肌萎缩的作用及机制[J]. 实验动物与比较医学, 2017, 37(3): 185-190.
[14]	张云丽, 王林, 刘铁民. PI3K/Akt和AMPK信号通路在运动诱导的啮齿动物骨骼肌内GLUT4转位和表达中的作用[J]. 实验动物与比较医学, 2017, 37(1): 76-82.
[15]	金子越, 方展强. 醋酸甲羟孕酮暴露致食蚊鱼的雄激素效应[J]. 实验动物与比较医学, 2016, 36(1): 6-7.