实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (6): 436-440.DOI: 10.3969/j.issn.1674-5817.2011.06.008

• 论著 • 上一篇    下一篇

树鼩断尾后运动及生长发育状况观察

李波1, 金会艳2, 陈丽玲1, 郑红3, 刘汝文1, 何保丽1, 沈培清3, 角建林1   

  1. 1.昆明医学院实验动物中心, 昆明 650031;
    2.昆明医学院机能实验中心, 昆明 650031;
    3.云南省实验动物管理办公室, 昆明 650031
  • 收稿日期:2011-04-25 出版日期:2011-12-25 发布日期:2011-12-25
  • 作者简介:李波(1979-),女,硕士,E-mail:libo0302@sina.com
  • 基金资助:
    国家科技支撑计划项目(2009BAI831302),南省教育厅科学研究基金项目(40411110),云南省科技基础条件平台建设计划项目(2006PT07-1)

Observation on Motions, Growth and Development after Tail Docking in Tree Shrew

LI Bo1, JIN Hui-yan2, CHEN Li-ling1, ZHENG Hong3, LIU Ru-wen1, HE Bao-li1, SHENG Pei-qing3, JIAO Jian-lin1   

  1. 1. Laboratory Animal Center ,Kunming Medical University, Kunming 650031, China;
    2. Department of Central Laboratory for Functional Experiment, Kunming Medical College, Kunming 650031, China;
    3. Office of Laboratory Animal Administration of Yunnan Province, Kunming 650031, China
  • Received:2011-04-25 Online:2011-12-25 Published:2011-12-25

摘要: 目的 探索性进行树鼩断尾试验,初步探讨断尾对实验室驯养树鼩运动状况及生长发育的影响。方法 选取45只树鼩随机均分成3组,组1(断尾后饲养3个月)与组2(断尾后饲养6个月)从第2、3尾椎或3、4尾椎间实施断尾手术,分别饲养3个月、6个月后,用生理仪测定树鼩腓肠肌肌张力增量,与组3(对照组)腓肠肌肌张力增量进行比较。光镜检查各组腓肠肌组织机构; 各组内动物雌雄以1∶1交配(每组雌雄各5只),观察记录树鼩受孕、产仔及离乳情况。结果 断尾树鼩全部存活; 组1和组2,与对照组腓肠肌肌张力增量比较差异显著(P<0.05); 各组树鼩实验结束体重与增量无显著性差异(P>0.05); 各组树鼩受孕率无显著性差异(P>0.05)。结论 断尾对实验室驯养树鼩的运动有一定抑制作用,断尾树鼩可以正常生长发育。

关键词: 树鼩, 断尾, 腓肠肌, 肌张力, 生长发育

Abstract: Objective To explore the tail docking experiment in tree shrews and to observe the effects on their motions and reproduction conditions in the laboratory domestication. Methods Forty-five tree shrews were chosen and randomly divided into three groups: tree shrews in group 1 (bred for three months after tail docking, n=15) and group 2 ( bred for six months after tail docking, n=15) are tailed between the second and the third caudal vertebra or between the third and the forth caudal vertebra. Group 3 is the blank control group (n=15). The increases of gastrocnemius muscle tension of group 1 and 2 were compared with group 3. The structures of gastrocnemius of each group were detected by light microscope. Tree shrews in each group mated 1∶1( five males and females each group). Pregnancy, breeding and weaning were observed and recorded. Result All the tailed tree shrews have survived. The difference in the increases of gastronomies muscle tension in group 1 and 2 is significant compared with the blank control group (P<0.05). There are no obvious differences in the weights of the tree shrews and the increase of weights (P>0.05). No differences in conception rate were observed (P>0.05). Conclusion Tail docking has a certain inhibitory effect on the tree shrews’ motions, but they can grow, breeding and develop normally.

Key words: Tree shrew, Tail docking, Gastrocnemius, Muscle tension, Growth and development

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