寒冷环境对犬腹部爆震伤后肝脏损伤的影响

doi:10.12300/j.issn.1674-5817.2025.190

摘要/Abstract

摘要：

【目的】 探究寒冷环境对犬腹部爆震伤后机体生理指标、动脉血气、肝功能及肝组织病理改变的影响。 【方法】 10只普通级比格犬，随机分为常温环境处理组、寒冷环境处理组，两组均使用多功能动物造模平台制备腹部爆震伤模型，造成肝脏损伤。模型建立后，常温环境处理组的比格犬置于常温环境中，寒冷环境处理组的比格犬置于-25 ℃的寒舱设施内。分别于爆震前24 h、爆震后即时，及爆震后40 min采集生命体征、动脉血气、肝功能数据，并对肝组织进行病理学分析。 【结果】 爆震前24 h及爆震后即时，两组各生理指标、动脉血气及肝功能差异均无统计学意义（P>0.05）；爆震后40 min，寒冷环境处理组心率、体温、收缩压（systolic blood pressure，SBP）显著异于常温环境处理组（P<0.05）；与常温环境处理组相比，寒冷环境处理组二氧化碳分压（partial pressure of carbon dioxide，PCO?）显著升高，碱剩余（base excess，BE）负值显著增加，且寒冷环境处理组总血清胆红素（total serum bilirubin，TSB）、间接胆红素（indirect bilirubin，IBIL）、丙氨酸氨基转移酶（alanine aminotransferase，ALT）和天冬氨酸氨基转移酶（aspartate aminotransferase，AST）水平明显升高（P<0.05）。肝组织HE染色结果显示，寒冷环境处理后，肝组织内出血情况更加严重，组织水肿更为突出，炎症反应更明显。 【结论】 寒冷环境会加剧腹部爆震伤后代谢性酸中毒的潜在趋势，加剧肝脏损伤。在寒冷环境创伤救治中，需加强体温管理、早期酸碱平衡干预及肝功能监测，以降低损伤风险。

关键词: 寒冷环境, 腹部爆震伤, 肝损伤, 比格犬

Abstract:

[Objective] To investigate the effects of cold environment on physiological parameters, arterial blood gas, liver function, and pathological changes of liver tissue in dogs after abdominal blast injury. [Methods] Ten conventional-grade Beagle dogs were randomly allocated to either a ambient temperature group or a cold exposure group. Both groups underwent abdominal blast injury modeling using a multifunctional animal modeling platform to induce liver injury. Following model establishment, dogs in the ambient temperature group were housed under ambient temperature conditions, while those in the cold exposure group were placed in a cold chamber facility maintained at -25℃. Vital signs, arterial blood gas, and liver function parameters were collected at 24 h before blast injury, immediately after blast injury, and 40 min after blast injury, respectively. Pathological analysis of liver tissues was subsequently conducted. [Results] At 24 h before blast injury and immediately after blast injury, no statistically significant differences were observed in physiological indicators, arterial blood gas, or liver function parameters between the two groups (P>0.05). At 40 min after blast injury, heart rate, body temperature, and systolic blood pressure (SBP) in the cold exposure group differed significantly from those in the ambient temperature group (P<0.05). Compared with the ambient temperature group, the cold exposure group showed a significant increase in partial pressure of carbon dioxide (PCO₂), a marked elevation in the negative value of base excess (BE), and significantly higher levels of total serum bilirubin (TSB), indirect bilirubin (IBIL), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) (P<0.05). Hematoxylin-eosin (HE) staining of liver tissues demonstrated that the cold exposure group presented more severe intrahepatic hemorrhage, more prominent tissue edema, and a more pronounced inflammatory response.. [Conclusion] The cold environment will exacerbate the potential trend of metabolic acidosis after abdominal blast injury and intensify liver damage. In the treatment of trauma in cold environments, it is necessary to strengthen body temperature management, conduct early intervention for acid - base balance, and monitor liver function to reduce the risk of injury.

Key words: Cold environment, Abdominal blast injury, Liver injury, Beagle dogs

中图分类号:

Q95-33

王馨培,宫田田,郭天革,等.寒冷环境对犬腹部爆震伤后肝脏损伤的影响[J]. 实验动物与比较医学.. DOI: 10.12300/j.issn.1674-5817.2025.190.

WANG Xinpei,GONG Tiantian,GUO Tiange,et al. Effects of Cold Environment on Liver Injury in Dogs after Abdominal Blast Injury[J]. Laboratory Animal and Comparative Medicine. DOI: 10.12300/j.issn.1674-5817.2025.190.

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