人脑胶质瘤大鼠移植模型的初步建立

doi:10.12300/j.issn.1674-5817.2020.094

实验动物与比较医学 ›› 2021, Vol. 41 ›› Issue (3): 220-225.DOI: 10.12300/j.issn.1674-5817.2020.094

• 论著:人类疾病动物模型 • 上一篇下一篇

人脑胶质瘤大鼠移植模型的初步建立

谭邓旭, 吴朋朋, 赵勇, 师长宏, 葛煦

空军军医大学实验动物中心,西安 710032

收稿日期:2020-07-14 修回日期:2020-12-03 出版日期:2021-06-25 发布日期:2021-07-05
作者简介:谭邓旭(1991—), 男, 本科, 助理实验师, 从事肿瘤模型建立及评价相关工作。E-mail: 494117685@qq.com
基金资助:
基础加强计划重点基础研究项目(2017-JCJQ-ZD-051-04); 军队实验动物专项课题[SYDW(2017)02号; SYDW (2018)01号]

Establishment of a Xenograft Model of Human Glioma in Rats

TAN Dengxu, WU Pengpeng, ZHAO Yong, SHI Changhong, GE Xu

Laboratory Animal Center, Air Force Medical University, Xi'an 710032, China

Received:2020-07-14 Revised:2020-12-03 Online:2021-06-25 Published:2021-07-05

摘要/Abstract

摘要： 目的利用免疫抑制剂初步建立人脑胶质瘤大鼠异种移植模型,为临床和基础研究提供理想的实验工具。方法选择临床常用免疫抑制剂雷帕霉素（rapamycin）和环孢霉素A（cyclosporin （A）,于手术前3 d开始给SD大鼠灌胃,分为雷帕霉素单药（Rapa）组、环孢霉素A单药（CsA）组、联合用药（Rapa+CsA）组及对照组。用人脑胶质瘤U87-MG细胞分别进行SD大鼠脑部原位移植和右侧背部皮下移植,监测大鼠肿瘤体积和体质量的变化。当肿瘤生长至大约600 mm³时,通过近红外荧光活体成像法检测大鼠原位脑胶质瘤生长情况;然后处死大鼠,分离组织标本,采用HE染色和免疫组织化学法检测肿瘤组织学形态。结果与Rapa组和CsA组相比,联合用药（Rapa+CsA）在免疫抑制方面表现出明显优势,模型成功率达到100%（Rapa和CsA单药的成模率分别为0%和40%）。联合用药组大鼠体质量明显小于Rapa组、CsA组和对照组。活体成像发现近红外荧光染料在大鼠脑部富集,相应的组织病理分析确定为脑胶质瘤。用人线粒体Mitochondria抗体进行的免疫组织化学染色结果显示肿瘤部位为强阳性。结论联合用药（Rapa+CsA）进行免疫抑制后接种人脑胶质瘤U87-MG细胞,可成功建立人脑胶质瘤大鼠异种移植模型,病理分析确认了人脑胶质瘤的组织形态。

关键词: 人脑胶质瘤, 雷帕霉素, 环孢霉素A, 活体成像, 异种移植

Abstract: Objective To establish a rat xenograft model of human glioma and to provide ideal animal models for clinical and basic research by injecting immunosuppressants. Methods SD rats were divided into rapamycin (Rapa), cyclosporin A (CsA), Rapa+CsA, or negative control groups (n = 5 each) for 3 days before surgery. Human glioma U87-MG cells were injected orthotopically into the rat brains and subcutaneously into the back, and the volume and weight of subcutaneous tumors were monitored regularly. Once the subcutaneous tumors had grown to approximately 600 mm³, the growth of the brain glial tumors in situ was detected by near-infrared fluorescence optical imaging. The rats were sacrificed and tissue samples were isolated, and hematoxylin and eosin and immunohistochemical (IHC) staining were performed to determine the modeling success rate. Results Compared with Rapa or CsA treatment, Rapa+CsA treatment showed an obvious immunosuppressive effect. The success rate of Rapa+CsA treatment model reached to 100%, while that of Rapa or CsA treatment model was only 0% and 40%, respectively. The mean body weight of the rats in the combination group was significantly lower than that in the other groups. A strong near-infrared fluorescent dye signal was detected in the rat brain by optical imaging and was further confirmed to be glioma tissue by histopathological analysis. The IHC staining was strongly positive for the human mitochondrial antibody at the tumor site. Conclusion A rat xenograft model of human glioma can be successfully established by the combined injection of immunosuppressants Rapa+CsA, and the morphology of human glioma can be conformed by pathological analysis.

Key words: Human glioma, Rapamycin, Cyclosporin A, Optical image, Xenograft

中图分类号:

Q95-33
R-332

谭邓旭, 吴朋朋, 赵勇, 师长宏, 葛煦. 人脑胶质瘤大鼠移植模型的初步建立[J]. 实验动物与比较医学, 2021, 41(3): 220-225.

TAN Dengxu, WU Pengpeng, ZHAO Yong, SHI Changhong, GE Xu. Establishment of a Xenograft Model of Human Glioma in Rats[J]. Laboratory Animal and Comparative Medicine, 2021, 41(3): 220-225.

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人脑胶质瘤大鼠移植模型的初步建立

Establishment of a Xenograft Model of Human Glioma in Rats

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