Compound Casson Syrup Alleviates Acute Chemical Liver Injury in Mice Caused by D-galactosamine

doi:10.12300/j.issn.1674-5817.2021.140

Abstract

Abstract:

Objective To investigate the protective effect of compound Casson syrup on acute chemical liver injury induced by D-galactose in mice.Methods The chemical liver injury model was established by intraperitoneal injection of D-galactose 800 mg/kg. The experiment was divided into 6 groups, including normal control group, model control group, positive control group of Yanxiaodinar syrup, and compound Casson syrup low-dose, medium-dose and high-dose groups, with 8 rats in each group. After intragastric administration of compound Casson syrup (8 mL·kg^-1·d^-1) and Yanchaudinar syrup (12 mL·kg^-1·d^-1), the changes of serum biochemistry, liver function and immunological indexes of liver injury were detected, and the pathological changes of liver tissue were observed.Results Compared with the normal control group, the liver index of model group was increased (P < 0.05), suggesting liver injury. Compared with model group, serum biochemical and liver function indexes were decreased after treatment with compound Casson syrup (P < 0.05). Compared with normal control group, the activities of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in liver homogenate of model group were increased (P < 0.05), and the activity of superoxide dismutase (SOD) was significantly decreased (P < 0.01). Compared with model group, SOD activity in compound Casson syrup groups was increased (P < 0.05). HE staining showed the inflammatory cell infiltration, degeneration, necrosis and other pathological phenomena of mouse liver caused by D-galactose were reversed after Casson syrup treatment.Conclusion Compound Casson syrup can effectively relieve the liver injury induced by D-galactose, and has a certain protective effect on liver.

Key words: Compound Casson syrup, Acute chemical liver injury, Liver protection, Mice

CLC Number:

R-332

·Ablimiti Merika,·Abibule Aishan,Shen SHI,et al. Compound Casson Syrup Alleviates Acute Chemical Liver Injury in Mice Caused by D-galactosamine[J]. Laboratory Animal and Comparative Medicine, 2022, 42(3): 194-200. DOI: 10.12300/j.issn.1674-5817.2021.140.

Figures/Tables 4

References 16

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组别 Group	剂量 Dose/(mL·kg^-1·d^-1)	实验前体质量 Pre-test body mass/g	解剖前体质量 Preanatomic body mass/g	肝脏指数 Liver index/ (mg·g^-1)	脾脏指数 Spleen index/ (mg·g^-1)
正常对照 Normal control	-	24.00±1.07	29.19±1.70	4.95±0.61	0.30±0.07
模型对照 Model control	-	25.00±2.39	28.85±4.88	5.93±0.84^*	0.36±0.01
炎消迪娜儿糖浆对照 YS control	12	20.13±3.31	27.16±2.98	4.42±0.75^#	0.32±0.05
低剂量复方卡森糖浆 Low-dose of CCS	4	27.50±0.76	30.99±1.36	4.50±0.38	0.26±0.05^#
中剂量复方卡森糖浆 Medium-dose of CCS	8	22.88±3.36	28.91±1.75	5.35±0.83	0.28±0.06^#
高剂量复方卡森糖浆 High-dose of CCS	16	26.13±0.99	28.66±1.81	4.64±0.29	0.32±0.06

组别 Group	剂量 Dose/(mL·kg^-1·d^-1)	实验前体质量 Pre-test body mass/g	解剖前体质量 Preanatomic body mass/g	肝脏指数 Liver index/ (mg·g^-1)	脾脏指数 Spleen index/ (mg·g^-1)
正常对照 Normal control	-	24.00±1.07	29.19±1.70	4.95±0.61	0.30±0.07
模型对照 Model control	-	25.00±2.39	28.85±4.88	5.93±0.84^*	0.36±0.01
炎消迪娜儿糖浆对照 YS control	12	20.13±3.31	27.16±2.98	4.42±0.75^#	0.32±0.05
低剂量复方卡森糖浆 Low-dose of CCS	4	27.50±0.76	30.99±1.36	4.50±0.38	0.26±0.05^#
中剂量复方卡森糖浆 Medium-dose of CCS	8	22.88±3.36	28.91±1.75	5.35±0.83	0.28±0.06^#
高剂量复方卡森糖浆 High-dose of CCS	16	26.13±0.99	28.66±1.81	4.64±0.29	0.32±0.06

组别 Group	剂量 Dose/(mL·kg^-1·d^-1)	ALT z/(U·L^-1)	AST z/(U·L^-1)	AST/ALT	ALP z/(U·L^-1)
正常对照 Normal control	-	34.63±1.41	144.50±27.30	4.18±0.84^#	171.50±20.10^#
模型对照 Model control	-	101.13±51.3^*	241.25±41.04^*	2.39±1.69	217.50±81.39^*
炎消迪娜儿糖浆对照 YS control	12	53.60±10.43	216.20±50.45	4.19±1.47	157.80±17.30^#
低剂量复方卡森糖浆 Low-dose of CCS	4	59.63±37.74	182.63±56.29	3.44±0.99	169.13±28.98^#
中剂量复方卡森糖浆 Medium-dose of CCS	8	45.25±17.80	159.13±31.84	3.79±0.95	173.38±27.64^#
高剂量复方卡森糖浆 High-dose of CCS	16	38.80±4.76	145.80±13.03	3.83±0.75	149.60±24.81^#

组别 Group	剂量 Dose/(mL·kg^-1·d^-1)	ALT z/(U·L^-1)	AST z/(U·L^-1)	AST/ALT	ALP z/(U·L^-1)
正常对照 Normal control	-	34.63±1.41	144.50±27.30	4.18±0.84^#	171.50±20.10^#
模型对照 Model control	-	101.13±51.3^*	241.25±41.04^*	2.39±1.69	217.50±81.39^*
炎消迪娜儿糖浆对照 YS control	12	53.60±10.43	216.20±50.45	4.19±1.47	157.80±17.30^#
低剂量复方卡森糖浆 Low-dose of CCS	4	59.63±37.74	182.63±56.29	3.44±0.99	169.13±28.98^#
中剂量复方卡森糖浆 Medium-dose of CCS	8	45.25±17.80	159.13±31.84	3.79±0.95	173.38±27.64^#
高剂量复方卡森糖浆 High-dose of CCS	16	38.80±4.76	145.80±13.03	3.83±0.75	149.60±24.81^#

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