Dynamic Observation on In vivo Bioluminescence Imaging of Experimental Metastatic Animal Models in Nude Mice

doi:10.3969/j.issn.1674-5817.2012.05.002

Abstract

Abstract: Objective To observe the tumor metastasis in deep organisms of the nude mice by in vivo bioluminescence imaging system. Methods The SMMC-7721-GFP/Luc cells with different concentrations were intravenously inoculated into the tail vein and spleen of the BALB/c-nu/nu mice, the distribution and expression of luciferase in nude mice were monitored by in vivo bioluminescence imaging system. Results The experimental metastatic animal models had been successfully established. The distribution and expression of luciferase ascended with cell concentration increased and decreased with the passage of time. Conclusion The in vivo bioluminescence imaging system may monitor the in vivo growth and metastasis of tumors and provide for studying the mechanisms of tumor metastasis and development of anticancer drug.

Key words: GFP/Luc double-tagging, Lentivirus carrier, In vivo bioluminescence imaging system, Metastasis, Animal model

CLC Number:

Q95-33

YAN Ming-xia, ZHU Miao-xin, LIU Lei, Li Jing, Lin He-chun, ZHAO Fang-yu, YAO Ming. Dynamic Observation on In vivo Bioluminescence Imaging of Experimental Metastatic Animal Models in Nude Mice[J]. Laboratory Animal and Comparative Medicine, 2012, 32(5): 376-382.

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