Comparative Observation on Three Kinds of Cranio-Orbital Injury Models in Rats

doi:10.3969/j.issn.1674-5817.2013.01.007

Abstract

Abstract: Objective To establish cranio-orbital injury models in rats which may help the research of the mechanisms and treatment of the injury. Methods Sixty rats were randomly divided into 3 groups, group A(forceps injury group),group B(free fall impact injury group) and group C(fluid percussion injury group). The cranio-orbital injury models on the left eyes of rats in the 3 groups were established respectively. The incidence of Marcus-gun pupil, exophthalmos, hypophasis, subhyaloid hemorrhage, optic nerve transaction, infection, orbit fracture, optic nerve sheaths hemorrhage, contusion and laceration of the brain in the model eyes and the mortality were counted respectively after surgery. The retina of normal eyes and model eyes were observed by microscope, the ultrastructure of retinal ganglion cells (RGCs) were observed under electron microscope. Results The performance of group A was superior to that of the other two groups with the standard of cranio-orbital injury models. The retina structure of model eyes was disorganized and the quantity of RGCs significantly reduced after injury. The ultrastructure of RGCs in model eyes showed the typical characteristics of apoptosis. Conclusion The animal models of cranio-orbital injury which according with the clinical reality can be established successfully with fluid percussion brain injury device (FPI) .This model provides a solid experimental foundation for future searching the mechanisms and treatment of the injury.

Key words: Cranio-Orbital Injury, Optic nerve, Animal model

CLC Number:

R774.6Q95-33

GAO Chen, ZHOU Hai-yan, LI Zhi-yun, ZHANG Jing-ke, JIA Pin, WANG Xiang-yang. Comparative Observation on Three Kinds of Cranio-Orbital Injury Models in Rats[J]. Laboratory Animal and Comparative Medicine, 2013, 33(1): 33-38.

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