Impact of Several Welfare Intervention Measures on Stress-related Index in Mice

doi:10.3969/j.issn.1674-5817.2014.06.007

Abstract

Abstract: Objective To study the impact of frequently-used material intervention measures towards stress-related index of laboratory animals. Methods Sixty weaning male KM mice were randomly assigned to five groups: A was routine feeding group, B nutritional intervention group, C sunflower seed group, D toy group, and E incense group, with 12 mice in each group. Food and water intake were measured every day. Body weight was measured every two days. Blood sample was collected 37 days later from the caudal vein in mouse tail and blood routine examination has been performed. mRNA index of GR, POMC, nNOS, BDNF, NMDA, TH and TPH in brain tissues of the mice were measured by fluorescent real-time PCR method and differences between material intervention groups and routine feeding group were compared. Results Compared with group A, the body weights of mice in other groups reduced. For food intake, group B reduced compared with group A, while group D and E increased. For water intake, group B reduced compared wtih group A, while group D and E increased. For the level of BDNF mRNA expression, comparing with group A, group F and E increased obviously, while group C reduced to some extent. For the level of GR mRNA expression, comparing with group A, group D reduced obviously, while group C and E reduced to some extent. For the level of POMC mRNA expression, comparing with group A, group D reduced to some extent, while other groups reduced significantly. For the level of NMDA mRNA expression, comparing with group A, group D increased obviously. For the level of TH mRNA expression, comparing with group A, group B and C reduced to some extent. For the level of TPH mRNA expression, comparing with group A, group C reduced to some extent. Hematological parameters had no obvious difference among all groups. Conclusions Placing toys makes food and water intake rise sharply, ability to resist fear and anxiety enhanced obviously, learning and memory ability reinforced greatly and stress-related index reduced evidently. Incense makes food and water intake go up, ability to resist fear and anxiety enhanced obviously and stress-related index reduced evidently .

Key words: Welfare intervention, Environmental enrichment, Mice, Stress parameter

CLC Number:

Q95-33

ZHOU Jia-liang, HU Ying, SHA Xiao-min, YANG Fei, AI Xiao-jie. Impact of Several Welfare Intervention Measures on Stress-related Index in Mice[J]. Laboratory Animal and Comparative Medicine, 2014, 34(6): 467-472.

References

[1] GB 14925-2010.实验动物环境及设施[S].
[2] Balcombe JP, Barnard ND, Sandusky C.Laboratory routines cause animal stress[J]. Lab Anim Sci, 2004, 43(6):42-51.
[3] Shaw D, Norwood K, Leslie JC.Chlordiazepoxide and lavender oil alter unconditioned anxiety-induced c-fos expression in the rat brain[J]. Behav Brain Res, 2011, 224(1):1-7.
[4] 胡樱, 杨斐. 小鼠短途运输及其营养干预的研究[J]. 实验动物科学, 2012, 29(3):15-19.
[5] 石瑜瑜, 傅佳, 操礼琼. 迷迭香精油通过嗅觉通路改善小鼠学习记忆的研究[J]. 现代药物与临床, 2012, 27(6):562-565.
[6] 周小江, 邵青, 张新创. 观察分析不同环境因素对小鼠应激反应的作用[J]. 微生物免疫学进展, 2013, 41(2):33-36.
[7] Leger M, Paizanis E, Dzahini K, et al. Environmental enrichment duration differentially affects behavior and neuroplasticity in adult mice[J]. Cereb Cortex,2014 Jun 5. pii: bhu119.
[8] Rivard GF, Moser EG, D'Ambrose SP, et al. Lack of fear response in mice (Mus musculus) exposed to human urine odor[J]. J Am Assoc Lab Anim Sci, 2014, 53(2):141-145.
[9] Stockner T, Sterk H, Kaptein R, et al.Molecular dynamics studies of a molecular switch in the glucocorticoid receptor[J]. J Mol Biol, 2003, 328(2):325-334.
[10] Yukihiro T, Toshihiro M, Chikara T.Glucocorticoid receptor α expression in the intrahepatic biliary epithelium and adjuvant steroid therapy in infants with biliary atresia[J]. J Pediatr Surg, 2005, 40(10):1574-1580.
[11] 程晓刚, 粟永萍, 罗成基. 糖皮质激素受体结构与功能研究进展[J]. 国外医学: 分子生物学分册, 2003, 25(10): 29-33.
[12] Santarelli S, Lesuis SL, Wang XD, et al.Evidence supporting the match/mismatch hypothesis of psychiatric disorders[J]. Eur Neuropsychopharmacol, 2014, 24(6):907-918.
[13] Bekinschtein P, Cammarota M, Katche C, et a1. BDNF is essential to promote persistence of long-term memory storage[J]. PNAS, 2008, 105(7): 2711-2716.
[14] Kempermann G.Regulation of adult hippocampal neurogenesis-implications for novel theories of major depression[J]. Bipolar Disord, 2002, 4(1):17-33
[15] Jacobs BL.Adult brain neurogenesis and depression[J].Brain Behav Immum, 2002, 16(5):602-609.
[16] Grégoire CA, Bonenfant D, Le Nguyen A, et al.Untangling the influences of voluntary running, environmental complexity, social housing and stress on adult hippocampal neurogenesis[J]. PLoS One, 2014, 9(1):e86237.
[17] Kaufman S.Tyrosine hydroxylase[J]. Adv Enzymol Relat Areas Mol Biol, 1995, 70:103-220.
[18] 白宝丰, 王斌, 张蕴琨. 游泳训练及环境应激对大鼠中脑酪氨酸羟化酶与纹状体多巴胺含量的影响[J]. 南京体育学院学报: 自然科学版, 2010, 9(3):1-3.
[19] Lobo MK, Zaman S, Damez-Werno DM, et al.ΔFosB induction in striatal medium spiny neuron subtypes in response to chronic pharmacological, emotional, and optogenetic stimuli[J]. J Neurosci, 2013, 33(47):18381-18395.
[20] Sapolsky RM.Glucocorticoids and hippocampal atrophy in neuropsychiatric disorders[J]. Arch Gen Psychiatry, 2000, 57(10):925-935.
[21] 杜雨苍. 神经肽与脑功能[M]. 上海: 上海科学技术教育出版社, 1998:151-171.