Microphthalmia Mutagenesis Mouse Induced by N-ethyl-N-nitrosourea and Its Genetic Tests

doi:10.3969/j.issn.1674-5817.2014.06.008

Abstract

Abstract: Objective To obtain the eye mutation mice induced by ethylnitrosure (ENU) which would offer the models for human eye diseases research. Methods N-ethyl-N-nitrosourea (ENU) was intraperitoneally injected in forty C57BL/6J (B6) male mice aged 8-10 weeks old. The male mice were mated with the same strain female mice, and their progenies were screened for visible eye mutation. Genetic tests were carried out. Results An inherited microphthalmia mouse was obtained by ENU-induced mutagenesis. Genetic tests showed that genetic background can affect the penetrance of the defect. The penetrance of observed eye defect were 90%, 0% and 27.8% on the B6, BDF1 and BDBN2, respectively. Conclusion The microphthalmia mice models was obtained by ENU-induced mutagenesis, it may be used for human microphthalmia disease research.

Key words: N-ethyl-N-nitrosourea (ENU), Microphthalmia, Mutagenesis, Mouse models

CLC Number:

Q95-33

WANG Jian-ming, GENG Teng, CHEN Hang, NI Jun-da, WANG Wen-hua, CHEN Bing, XUE Zheng-feng. Microphthalmia Mutagenesis Mouse Induced by N-ethyl-N-nitrosourea and Its Genetic Tests[J]. Laboratory Animal and Comparative Medicine, 2014, 34(6): 473-475.

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