Abstract: Objective To establish the IL-1β-induced recurrence model in rat with acetic acid-induced gastric ulcer and cured by Omeprazole. Methods 1) The acetic acid-induced gastric ulcer rat model was reproduced by topical application of acetic acid, an improved method of Okabe. 2) Then, the rats with gastric ulcer were divided into 2 groups randomly, the rats of control group (spontaneous closure group) were intragastric administrated with physiological saline, while the rats of therapy group were administrated with Omeprazole. The rats Randomly sampled on the 14th, 21th and 28th day after treatment, together with the rats on the 28th day in control group, were executed at one time. Then the care rates(%), ulcer index, and the histopathological changes of gastric mucosa were assessed. 3) The cured rats were randomly divided into 2 groups, and were intraperitoneally injected with IL-1β of 0.75 mg/kg and 1.00 mg/kg respectively. The recurrence rate of ulcer (%), ulcer index, and histopathological changes of gastric mucosa were assessed 48h later. Results 1) The model rats induced by acetic acid showed typical ulcer when dissected randomly for observation. 2) After 14, 21 and 28 days’ administration of Omeprazole, the cure rates were 25.0%, 37.50% and 100.0%, respectively. The thickness of regenerative gastric mucosa increased with the extension of therapy time. While the number of cystic-dilated glands, inflammatory cells and capillary vessels reduced as the treatment time increased. The cure rate of spontaneous closure with 28 days was 37.50%. 3) In the IL-1β-1.00 mg/kg group, the recurrence rate of ulcer was 100%, and the ulcer index was 18.0±3.4. Moreover, compared with rats 28 days after treatment, the regenerative gastric mucosa was significantly thinned, but the number of cystic-dilated glands, inflammatory cells and capillary vessels obviously increased. Conclusion The model of interleukin1β-induced recrudescence model in rat with acetic acid-induced gastric ulcer which has been treated by Omeprazole was applicable to the study of prevention and treatment of recurrence of peptic ulcer.

Key words: Il-1β, Gastric ulcer recurrence, Rat, Omeprazole