Pathologic Diagnosis of a Pituicytoma in a Han-Wistar Rat

doi:10.12300/j.issn.1674-5817.2023.064

Abstract

Abstract:

A case of pituicytoma was observed in a Han-Wistar rat from the control group of a 2-year carcinogenicity study. No obvious abnormality were found in clinical observation and necropsy. Hematoxylin and Eosin (HE) staining results showed that nodular hyperplasia in the pars nervosa of the pituitary, which was well demarcated and compressed the adjacent normal tissue. The tumor cells were similar to the glial cells with round or oval nuclei, cytoplasm rich in eosinophilic or vacuole. The tumor cells differentiated well, with no obvious cell pleomorphism and visible mitotic figures. Some tumor cells were arranged in a pseudorosette formation. Immunohistochemical staining (IHC) analysis confirmed positive expression of Glial fibrillary acidic protein (GFAP) and S-100 protein. The tumor was diagnosed as the spontaneous benign pituicytoma combining the HE and IHC staining results.

Key words: Pituicytoma, Han-Wistar rat, Pathological diagnosis

CLC Number:

R739.4

Minbo HOU, Tiantian CUI, Naying SU, Miaomiao ZHANG, Yongmin JIAO, Jianyan YAN, Xijie WANG, Ohira TOKO. Pathologic Diagnosis of a Pituicytoma in a Han-Wistar Rat[J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 654-658.

Figures/Tables 2

Figure 1 Histopathological results of a the tumor from a Han-Wistar rat （HE staining）Note：A, The well demarcated mass in the pars nervosa of the pituitary (scale bar: 500 μm); B, The mass compressed the adjacent neurohypophysis and the pars intermedia (scale bar: 200 μm); C, The tumor cells were similar to the glial cells (scale bar: 100 μm); D, Some tumor cells were arranged in a pseudorosette formation (scale bar: 100 μm); E, The magnification of figure D (scale bar: 50 μm); F, The cytoplasm of tumor cells were rich in eosinophilic or vacuolate. Microglial cells-like tumor cells (black arrow) and mitotic figures (yellow arrow) were present (scale bar: 50 μm).

Figure 2 Immunohistochemistry staining of tumor from a Han-Wistar rat （DAB staining）Note：A-B， Positive immunohistochemical reaction with glial fibrillary acidic protein （GFAP）（scale bar： 200 μm or 100 μm）； C， Positive immunohistochemical reaction with S-100 protein （scale bar： 100 μm or 50 μm）.

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