Abstract: Objective To observe the changes of the behavior and serum CORT level, activity of TChE and content of 5-HT in brain tissue of sub-health state rat model induced by restraint stress, and to explore the effect of the Traditional Chinese Medicine and its mechanism. Methods Fourty male SD rats weighted 320-360 g were randomly divided into 4 groups: normal control group, model control group, Anti-ageing tablets group and Liuwei Dihuang pills group, 10 rats per group. The sub-health state rats were induced by restraint which simulating human physiological stress. At the same time, the rats in Anti-ageing tablets group and Liuwei Dihuang pills group were administrated by Anti-ageing tablets and Liuwei Dihuang pills. During the experiment, endurance capacity, behavioral memory ability ,serum CORT level，activity of TChE and content of 5-HT in brain tissue of sub-health state rats were determined.Results Compared with normal control group, the struggle times of the rats in the model control group rcduccd obviously while the still time prolonged (P<0.01). The swimming distance and the latcncy period of searching for the plat remarkably prolonged (P<0.05); The latency period of active avoidance response,electric shock times and electric shock time had an increasing trend. Serum CORT level，activity of TChE and content of 5-HT in brain tissue of the rats obviously increased (P<0.05). After treatment of Anti-aging tablets and Liuwei Dihuang pills, the rats struggled more seriously, the still time presented a decreasing trend, especially the rat treated wi of searching for the plat had a decreasing trend (P<0.01).The latency period of active avoidance response, electric shock times and electric shock time had no obvious difference; the scrum CORT level, activity of TChE and cth Anti-ageing tablets (P<0.01); The swimming distance was decreased (P<0.05); the latency periodontent of 5-HT in brain tissue decreased remarkably (尸<0.05，P<0.01). Conclusion The endurance capacity and the learning and memory ability of the sub-health state rat induced by restraint stress was decreased, the serum CORT level, activity of TChE and content of 5-HT in brain tissue was increased remarkably; Anti-ageing tablets and Liuwei Dihuang pills could improve the behavior of sub-health state rat, and the mechanism may be related to regulate hormone content and neurotransmitter function.

Key words: Restraint stress, Sub-health state, Behavior, CORT, TChE, 5-HT;Traditional Chinese Medicine