Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model

doi:10.12300/j.issn.1674-5817.2021.095

Abstract

Abstract:

Objective To investigate the two different methods of making animal models of focal cerebral infarction by photochemical method and wire embolization method, and to analyze the correlation between infarct volume and behavior of experimental animals due to different modeling methods. Methods Totally sixty-four male Sprague-Dawley rats of 300-330 g were randomly divided into three groups: normal group, photochemical group and middle cerebral artery occlusion (MCAO) group. The neurological function scores and behavioral tests on the rats were performed 24 hours after surgery. Then brain tissues were removed by anesthesia and execution, and 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed to assess the brain infarct volume in rats. Results There were different volume infarct foci in the brain of SD rats in photochemical group and MCAO group. In MCAO model, most of the hemispheric cortex, striatum, thalamus, hippocampus and subventricular zone were involved in ischemia, and there were good correlations of neurological function scores and balance beam test scores with infarct volumes. In the photochemical model, the infarct foci were in cerebral cortex, and the neurobehavioral abnormalities were well correlated with the cortical infarct location, but the correlation with the infarct volume was relatively poor. Conclusion In MCAO modeling experiment, the behavioral outcomes closely correlated with infarct volume size, while the photochemical model can produce site-specific lesions and site-specific behavioral changed, and the behavioral outcomes have poor correlation with infarct volume.

Key words: Animal model of focal cerebral infarction, Wire embolization method, Photochemical method, Behavior, Infarct volume

CLC Number:

R-332

Huijuan LOU, Hongshi ZHANG, Yufeng WANG, Meng JIA, Deyu CONG. Comparative Study on the Presentation Effect of Photochemical Method and Wire Embolization Method for Making Focal Cerebral Infarction Model[J]. Laboratory Animal and Comparative Medicine, 2022, 42(1): 48-53.

Figures/Tables 2

References 25

1	HERPICH F, RINCON F. Management of acute ischemic stroke[J]. Crit Care Med, 2020, 48(11):1654-1663. DOI:10.1097/CCM.0000000000004597 .
2	SOMMER C J. Ischemic stroke: experimental models and reality[J]. Acta Neuropathol, 2017, 133(2):245-261. DOI:10.1007/s00401-017-1667-0 .
3	SINGH D, WASAN H, REETA K H. Preclinical stroke research and translational failure: a bird's eye view on preventable variables[J]. Cell Mol Neurobiol, 2021:2021 Mar 30. DOI:10.1007/s10571-021-01083-6 .
4	YAN T, CHOPP M, CHEN J L. Experimental animal models and inflammatory cellular changes in cerebral ischemic and hemorrhagic stroke[J]. Neurosci Bull, 2015, 31(6):717-734. DOI:10.1007/s12264-015-1567-z .
5	DURUKAN A, TATLISUMAK T. Acute ischemic stroke: overview of major experimental rodent models, pathophysiology, and therapy of focal cerebral ischemia[J]. Pharmacol Biochem Behav, 2007, 87(1):179-197. DOI:10.1016/j.pbb.2007.04.015 .
6	MCCABE C, ARROJA M M, REID E, et al. Animal models of ischaemic stroke and characterisation of the ischaemic penumbra[J]. Neuropharmacology, 2018, 134(Pt B):169-177. DOI:10.1016/j.neuropharm.2017.09.022 .
7	YANG Y, KIMURA-OHBA S, THOMPSON J, et al. Rodent models of vascular cognitive impairment[J]. Transl Stroke Res, 2016, 7(5):407-414. DOI:10.1007/s12975-016-0486-2 .
8	FLURI F, SCHUHMANN M K, KLEINSCHNITZ C. Animal models of ischemic stroke and their application in clinical research[J]. Drug Des Devel Ther, 2015, 9:3445-3454. DOI:10.2147/DDDT.S56071 .
9	MACRAE I M. Preclinical stroke research: advantages and disadvantages of the most common rodent models of focal ischaemia[J]. Br J Pharmacol, 2011, 164(4):1062-1078. DOI:10.1111/j.1476-5381.2011.01398.x .
10	SHAHI M, ABEDELAHI A, MOHAMMADNEJAD D, et al. Exact location of sensorimotor cortex injury after photochemical modulation; evidence of stroke based on stereological and morphometric studies in mice[J]. Lasers Med Sci, 2021, 36(1):91-98. DOI:10.1007/s10103-020-03017-y .
11	KHATEEB K, YAO Z J, KHARAZIA V N, et al. A practical method for creating targeted focal ischemic stroke in the cortex of nonhuman Primates[J]. Annu Int Conf IEEE Eng Med Biol Soc, 2019, 2019:3515-3518. DOI:10.1109/EMBC.2 019.8857741 .
12	LIU N W, KE C C, ZHAO Y H, et al. Evolutional characterization of photochemically induced stroke in rats: a multimodality imaging and molecular biological study[J]. Transl Stroke Res, 2017, 8(3):244-256. DOI:10.1007/s12975-016-0512-4 .
13	丁惠洁, 刘珂舟, 杨珊珊, 等. 大鼠脑皮层血管栓塞和再生过程的实时动态记录方法[J]. 生理学报, 2019, 71(4):581-587. DOI:10.13294/j.aps.2019.0015 .
14	YAO Z J, YAZDAN-SHAHMORAD A. A quantitative model for estimating the scale of photochemically induced ischemic stroke[J]. Annu Int Conf IEEE Eng Med Biol Soc, 2018, 2018:2744-2747. DOI:10.1109/EMBC.2018.8512880 .
15	MA D C, ZHANG N N, ZHANG Y N, et al. Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro [J]. J Ethnopharmacol, 2021, 270:113776. DOI:10.1016/j.jep. 2021.113776 .
16	WANG F J, LI R Y, TU P F, et al. Total glycosides of Cistanche deserticola promote neurological function recovery by inducing neurovascular regeneration via nrf-2/keap-1 pathway in MCAO/R rats[J]. Front Pharmacol, 2020, 11:236. DOI:10.3389/fphar.2020.00236 .
17	XIAO X T, LUO C, YUAN Y, et al. Systematic evaluation during early-phase ischemia predicts outcomes in middle cerebral artery occlusion mice[J]. Neuroreport, 2021, 32(1):29-37. DOI:10.1097/WNR.0000000000001553 .
18	TANG Y N, ZHANG G F, CHEN H L, et al. Selective brain hypothermia-induced neuroprotection against focal cerebral ischemia/reperfusion injury is associated with Fis1 inhibition[J]. Neural Regen Res, 2020, 15(5):903-911. DOI:10.4103/1673-5374.268973 .
19	GAO B Y, SUN C C, XIA G H, et al. Paired associated magnetic stimulation promotes neural repair in the rat middle cerebral artery occlusion model of stroke[J]. Neural Regen Res, 2020, 15(11):2047-2056. DOI:10.4103/1673-5374.282266 .
20	CHEN K H, CHAI H T, CHEN C H, et al. Synergic effect of combined cyclosporin and melatonin protects the brain against acute ischemic reperfusion injury[J]. Biomed Pharmacother, 2021, 136:111266. DOI:10.1016/j.biopha. 2021.111266 .
21	卢宝全, 尚小明, 杨峰, 等. 应用手持激光器建立光化学法脑梗死动物模型[J]. 中国比较医学杂志, 2012, 22(9):63-67, 80, 后插4. DOI:10.3969/j.issn.1671-7856.2012.009.014 .
22	VITAL S A, GAVINS F N E. Surgical approach for middle cerebral artery occlusion and reperfusion induced stroke in mice[J]. JoVE, 2016(116):54302. DOI:10.3791/54302 .
23	TAKANO K, TATLISUMAK T, BERGMANN A G, et al. Reproducibility and reliability of middle cerebral artery occlusion using a silicone-coated suture (Koizumi) in rats[J]. J Neurol Sci, 1997, 153(1):8-11. DOI:10.1016/s0022-510x(97)00184-6 .
24	WATSON B D, DIETRICH W D, BUSTO R, et al. Induction of reproducible brain infarction by photochemically initiated thrombosis[J]. Ann Neurol, 1985, 17(5):497-504. DOI:10.1002/ana.410170513 .
25	马浚宁, 高俊玮, 侯博儒, 等. 光化学栓塞法建立缺血性脑卒中动物模型[J]. 中国组织工程研究, 2015, 19(49):7951-7957. DOI:10.3969/j.issn.2095-4344.2015.49.014 .

[1]	Ling HU, Zhibin HU, Yunqing HU, Yuqiang DING. Overview of Studies in Animal Models of Schizophrenia [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 145-155.
[2]	Chaochao ZHANG, Xuesong TIAN. Application of Virtual Reality in the Behavior Analysis of Laboratory Animal [J]. Laboratory Animal and Comparative Medicine, 2023, 43(1): 73-78.
[3]	Sijia ZHAO, Xinyu HE, Quan JING, Lin MA, Chunlan GUO, Kuo WAN. Evaluation of Pain in Acute Pulpitis Hyperalgesia Model Rats [J]. Laboratory Animal and Comparative Medicine, 2022, 42(4): 333-341.
[4]	ZHANG Yan, YAN Shunsheng. Construction and Preliminary Application of a Rncat Congenital Cataract Mouse Model [J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 501-504.
[5]	HU Zhibin, HUANG Ying, DING Yuqiang. Construction and Evaluation of Animal Models for Cerebral Ischemia [J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 271-283.
[6]	YAN Yitong, BAI Fan, JING Yingli, WANG Limiao, LI Zihan, YU Yan. A Comparative Study on Spontaneous Homecage Behaviors of Spinal Cord Injury Mice [J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 290-298.
[7]	WAN Ying-han, SHEN Rui-ling, WANG Jue, GONG Hui, LI Jun, DONG Ru, FEI Jian. Comparative Studies on Behavior of C57BL/6J、129/SvJ and Their Hybrid Mice [J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 429-436.
[8]	WANG Yuan-yuan, DENG Wei-ping, LI Xiao-qiang, PENG Jia-xia. Experimental Observation on Effect of Astragalus Polysaccharides on Learning Ability in Rats with Post-stroke Depression [J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 443-448.
[9]	LIU Bo, WANG Yang, LI Zhi-jie. An Modified Eight-arm Maze Experiment Method in Behavioral Research [J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 226-230.
[10]	ZHANG Zhi-cheng, SONG Qing-kai, LI Xiao-hui, LI Na, YUAN Yuan, HUANG Xin, WANG Xuan, LI Ming-xue, DAI Jie-jie. Behavior Detection Method of MPTP Induced Tree Shrew Parkinson’s Disease Models [J]. Laboratory Animal and Comparative Medicine, 2019, 39(1): 9-14.
[11]	TIAN Li-li, WEI Xiao-feng, GAO Cheng. Progress in Research of Laboratory Animal Behavior and Environment [J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 237-242.
[12]	WANG Xiao-jie, ZHANG Zhi-jun, YE Dong-qing. Genetic Engineering Mice Models of Depressive-like or Depression-resistant Phenotype [J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 142-148.
[13]	LI Xia, WU Cheng-ye, XIAO Shi-fu, ZHENG Xian-jie, DONG Xiao-wei, PENG Su-fang, WANG Tao, XIAO Ze-ping. Chronic Unpredictable Mild Stressed Rats Increased Their Movements in Open Field [J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 175-181.
[14]	XIAO Hui,LENG Xiao-xia,CHEN Min-li,ZHU Ke-yan,ZHOU Wei-min,TAO Tao,PAN Yong-ming,SHOU Qi-yang. Behavior Changes of Rat in Sub-health State Induced by Restraint Stress and Altered by Traditional Chinese Medicine [J]. , 2010, 30(2): 124-128.
[15]	SHEN Xin-e1,WANG Guo-qing1,ZHOU Xi-ping1,ZOU Rong1,MA Tan-geng2. Effect of μ-Opioid Receptor Antisense Oligodeoxynucleotide on Methamphetamine-Induced Behavioral Sensitization in Mice [J]. , 2007, 27(3): 155-159.