Abstract: Objective To analyse genetic polymorphisms and genetic stability in mitochondrial ATPase6 and D-Loop gene of Closed Colony mice. Methods Twenty male ICR mice from 1000 mice of stochastic colony (offspring of 100 female parent mice) were studied, mitochondria ATPase6 gene and D-Loop gene of them were amplified respectively by using PCR of two pairs primers. The results from DNA sequencing were compared with the standard sequence of mice. Results The sequence of mtATPase6 gene and D-Loop gene was same in twenty ICR mice. Conclusion Polymorphismof mitochondrial DNA was not found. The ICR mice colony showed good genetic stability.

Key words: Closed Colony mice, mitochondria, ATPase6, D-Loop, genetic stability