Analysis of the Polymorphism of Mitochondrial DNA Control Region in Three Closed Colonies of Rabbits

doi:10.3969/j.issn.1674-5817.2014.01.006

Abstract

Abstract: Objective To discuss the genetic diversity and uniformity in three groups of closed colony rabbits based on mitochondrial DNA control region. Methods Seventy-one blood samples from New Zealand rabbit, Japanese White rabbit and Chinchilla rabbit were collected for amplifying ,purifying and sequencing the part of sequences of mitochondrial DNA control region by PCR and Genetic Analyzer. The variation sites, Parsimony informative sites, haplotypes, haplotype diversity, nucleotide diversity were analyzed by Mega 4.0 and DNA SP. Results 6 diversity sites and 9 haplotypes were found in the seventy-one rabbits, and most mutations were C→G. Every population had its own haplotypes. The polymorphism of Mitochondrial DNA Control Region and population diversity in three group were both low. Conclusions More sequence information about mtDNA sequences and population structure of laboratory rabbits were supplied,which was helpful to genetic breeding.

Key words: Closed colony rabbits, Mitochondrial DNA, Haplotype, Genetic polymorphism

CLC Number:

Q95-33

SHEN Xing-jiao, YUE Bing-fei, MA Li-ying. Analysis of the Polymorphism of Mitochondrial DNA Control Region in Three Closed Colonies of Rabbits[J]. Laboratory Animal and Comparative Medicine, 2014, 34(1): 29-34.

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