Sequence Analysis on Complete Mitochondrial Genome and Phylogeny of Microtus fortis fortis

doi:10.3969/j.issn.1674-5817.2013.03.001

Abstract

Abstract: Objective To obtain the nucleotide sequence of complete mitochondrial genome sequence of Microtus fortis fortis to provide molecular data for the genetic structure and phylogeny research of Microtus.Fortis. Methods By means of conventional and long distance PCR and sequence with the “primer walking” strategy to obtain the complete mitochondrial genome of M.f.fortis (Genbank: JF261174).The phylogenetic tree was constructed based on Cyt b gene to investigate the phylogenetic position of M.f.fortis. Results The length of mitochondrial genome of M.f.fortis is 16312bp, include 13 protein coding genes, 2 ribosomal RNAs, 22 transfer RNAs and one major noncoding region (CR region). The extended termination associated sequences (ETAS-1 and ETAS-2), conserved sequence block 1 (CSB-1) and a Poly(C)₁₀section were found in the CR region. The putative origin of replication for the light strand (O_L) of M.f.fortis showed high conservation in stem and adjacent sequences, but the difference existed in the loop region among different species and subspecies. Phylogenetic analysis results based on the cytochrome b gene showed the closest phylogenetic relationship with Microtus middendorffi in the genus Microtus. Conclusion The mitochondrial genome sequence of M.f.fortis showed a typical vertebrate pattern. This study can provide useful molecular data for the further study about phylogenic relationships and subspecies taxonomy of M.fortis in the future

Key words: Microtus.fortis.fortis, Mitochondrial genome, Phylogenetic relationship

CLC Number:

Q95-33

GAO Jun, NI Li-ju, SUN Feng-ping, WANG Jin-xiang, HU Jian-hua, GAO Cheng, LI Kai, XIAO Jun-hua, ZHOU Yu-xun. Sequence Analysis on Complete Mitochondrial Genome and Phylogeny of Microtus fortis fortis[J]. Laboratory Animal and Comparative Medicine, 2013, 33(3): 167-173.

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