Analysis of Goiter in Zebrafish

doi:10.3969/j.issn.1674-5817.2019.06.007

Abstract

Abstract: Objective To investigate the causes of goiter in zebrafish, and explore whether the disease is caused by iodine deficiency. Methods The enlarged tissue in the lower jaw of zebrafish was observed through HE staining and electron microscopy. Meanwhile, both iodine-fed and iodine-free feeding groups were set up, and the expressions of five thyroid-related genes were detected at different periods. Result According to HE staining, goiter was confirmed. Through electron microscopy observation, the mitochondria was enlarged and lysosomes was increased in the diseased tissues. The expression of corticotropinreleasing factor (CRF) was different between the two groups at 7 days post fertilization (7dpf). The expression of CRF in iodine group was significantly higher than that in non-iodine group. Conclusion Iodine deficiency can cause goiter in zebrafish,iodine deficiency causes a decrease in CRF expression.

Key words: Zebrafish, Goiter, Mitochondria

CLC Number:

Q95-33

Li Ying-niang, Dai Wei, Sheng Jian. Analysis of Goiter in Zebrafish[J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 462-466.

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