Laboratory Animal and Comparative Medicine ›› 2011, Vol. 31 ›› Issue (3): 207-210.DOI: 10.3969/j.issn.1674-5817.2011.03.011

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Preliminary Study on mtDNA D-loop of Yunnan Diannan Small-ear Pigs and Guangxi Bama Miniature Pigs

NI Li-ju1,2, XIE Jian-yun1, HU Jian-hua1   

  1. 1. Shanghai Quality Monitoring Centre for Laboratory Animals;
    2. Shanghai Laboratory Animal Research Center, Shanghai 201203, China
  • Received:2010-08-20 Online:2011-06-25 Published:2011-06-25

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Abstract: Objective To research the polymorphism of mtDNA in the outbred Diannan small-ear pigs and Guangxi Bama miniature pigs, and to discuss the methods of genetic monitoring on the miniature pigs. Methods The mtDNA D-loop of Diannan small-ear pigs and Guangxi Bama miniature pigs were amplified and analyzed using 18 restriction edonucleases by RFLP. Results The restriction cleavage patterns of the mtDNA D-loop showed monomorphism among or within the breeds of Diannan small-ear pigs and Guangxi Bama miniature pigs. Conclusion The genetic diversity of Diannan small-ear pigs and Guangxi Bama miniature pigs was poor, and the mtDNA PCR-RFLP techniques couldn't be applied to genetic monitoring of them.

Key words: Diannan small-ear pigs, Guangxi Bama miniature pigs, mtDNA D-loop, Polymorphism, PCR-RFLP

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